Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders
Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders
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DOI：
10.1136/bjo.2003.038497
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发表时间：
2004-10-01
影响因子：
4.1
通讯作者：
Kinoshita, S
中科院分区：
文献类型：
--
作者：
Nakamura, T;Inatomi, T;Kinoshita, S
背景/目的：确定严重眼表疾病患者培养的自体口腔上皮细胞移植的结果。方法：对四名史蒂文斯-约翰逊综合征（三只眼睛）或化学烧伤（三只眼睛）患者的眼睛（n = 6）进行研究。自体口腔上皮细胞在存在 3T3 成纤维细胞的情况下在裸露的羊膜载体上生长 2 - 3 周，然后进行空气提升。将所得片材移植到受损的眼睛上，并在手术后48小时确认角膜表面对片材的接受。评估眼表重建的成功率、移植物存活率、视力变化和术后并发症，并通过组织学评价培养的口腔上皮片的质量。结果：移植后48小时，所有6只眼的整个角膜表面均无上皮缺损，表明移植的口腔上皮完全存活。所有眼睛的视力均得到改善。在随访期间（平均13.8（SD 2.9）个月），角膜表面保持稳定，尽管所有眼睛都表现出轻度的周围新生血管形成。结论：在剥脱的羊膜上生长的自体口腔上皮细胞可以移植来治疗严重的眼表疾病。
Background/aims: To determine outcomes of transplants of cultivated autologous oral epithelial cells in patients with severe ocular surface disorders.Methods: The eyes ( n = 6) of four patients with Stevens-Johnson syndrome ( three eyes) or chemical burns ( three eyes) were studied. Autologous oral epithelial cells, grown for 2 - 3 weeks on a denuded amniotic membrane carrier in the presence of 3T3 fibroblasts, were air lifted. The resultant sheet was transplanted onto the damaged eye, and acceptance of the sheet by the corneal surface was confirmed 48 hours after surgery. The success of ocular surface reconstruction, graft survival, changes in visual acuity, and postoperative complications were assessed and the quality of the cultivated oral epithelial sheet was evaluated histologically.Results: At 48 hours after transplant, the entire corneal surface of all six eyes was free of epithelial defects indicating complete survival of the transplanted oral epithelium. Visual acuity was improved in all eyes. During follow up ( mean 13.8 (SD 2.9) months), the corneal surface remained stable, although all eyes manifested mild peripheral neovascularisation.Conclusions: Autologous oral epithelial cells grown on denuded amniotic membrane can be transplanted to treat severe ocular surface disorders.