Prevalence and mechanism of resistance against macrolides and lincosamides in Streptococcus suis isolates
Prevalence and mechanism of resistance against macrolides and lincosamides in Streptococcus suis isolates
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DOI：
10.1016/s0378-1135(01)00426-6
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发表时间：
2001-11-26
影响因子：
3.3
通讯作者：
Haesebrouck, F
中科院分区：
文献类型：
--
作者：
Martel, A;Baele, M;Haesebrouck, F
1999-2000 年从来自不同农场的病猪中回收的 87 株猪链球菌分离株，通过纸片扩散和琼脂稀释试验以及 PCR 检测，扩增 ermB 基因和 mefA/E 基因，筛选了对大环内酯类和林可酰胺类抗生素的耐药性。 71% 的分离株表现出对大环内酯类和林可酰胺类抗生素的组成型耐药性（MLSB 表型）。所有这些分离株在 PCR 中均呈 ermB 基因阳性，但 mefA/E 基因呈阴性。确定了所有菌株对其他五种抗菌剂的最低抑菌浓度 (MIC)。所有菌株均对青霉素敏感。百分之九十九的分离株对恩诺沙星和泰妙菌素敏感。百分之八十五的菌株对多西环素有抗药性。对八种猪链球菌菌株的 ermB 基因的 540 bp 片段进行了测序，并与五种肺炎链球菌和五种人类来源的化脓性链球菌菌株的 ermB 基因进行了比较。在 7 种猪链球菌、1 种肺炎链球菌和 3 种化脓性链球菌分离株中发现这些片段之间存在 100% 同源性。这项研究表明，对大环内酯类、林可酰胺类和链球菌素 B 的耐药性在猪链球菌中广泛存在，并由 el-MB 基因编码的核糖体甲基化介导。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Eighty-seven Streptococcus suis isolates recovered in 1999-2000 from diseased pigs, all from different farms, were screened for resistance against macrolide and lincosamide antibiotics by the disk diffusion and agar dilution test and a PCR assay, amplifying the ermB gene and the mefA/E gene. Seventy-one percent of the isolates showed constitutive resistance to macrolide and lincosamide antibiotics (MLSB-phenotype). All these isolates were positive for the ermB gene in the PCR, but negative for the mefA/E gene. For all strains minimum inhibitory concentrations (MIC) against five other antimicrobial agents were determined. All strains were susceptible to penicillin. Ninety-nine percent of the isolates were susceptible to enrofloxacin and tiamulin. Eighty-five percent of the strains were resistant to doxycycline. A 540 bp fragment of the ermB genes of eight S. suis strains was sequenced and compared with ermB genes of five S. pneumoniae and five S. pyogenes strains of human origin. A 100% homology was found between these fragments in seven S. suis, one S. pneumoniae and three of the S. pyogenes isolates.This study demonstrates that resistance against macrolides, lincosamides and streptogramin B is widespread in S. suis and mediated by ribosome methylation, encoded by the el-MB gene. (C) 2001 Elsevier Science B.V. All rights reserved.