FosB, a cysteine-dependent fosfomycin resistance protein under the control of σW, an extracytoplasmic-function σ factor in Bacillus subtilis
FosB, a cysteine-dependent fosfomycin resistance protein under the control of σW, an extracytoplasmic-function σ factor in Bacillus subtilis
复制标题
DOI：
10.1128/jb.183.7.2380-2383.2001
复制
发表时间：
2001-04-01
影响因子：
3.2
通讯作者：
Helmann, JD
中科院分区：
文献类型：
--
作者：
Cao, M;Bernat, BA;Helmann, JD
我们证明枯草芽孢杆菌FosB(YndN)基因编码一种磷霉素抗性蛋白。FosB的表达需要Sigma(W)，并且FosB和SigW突变体都对磷霉素敏感。FosB是一种金属硫醇转移酶，与依赖于Mn2+的谷胱甘肽转移酶的FOSA类相关，但偏爱镁和L半胱氨酸作为辅因子。
We demonstrate that the Bacillus subtilis fosB(yndN) gene encodes a fosfomycin resistance protein. Expression of fosB requires sigma (W), and both fosB and sigW mutants are fosfomycin sensitive. FosB is a metallothiol transferase related to the FosA class of Mn2+-dependent glutathione transferases but with a preference for Mg2+ and L-cysteine as cofactors.