Natural Anaplasma phagocytophilum infection of ticks and rodents from a forest area of Jilin Province, China
Natural Anaplasma phagocytophilum infection of ticks and rodents from a forest area of Jilin Province, China
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DOI：
10.4269/ajtmh.2006.75.664
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发表时间：
2006-10-01
影响因子：
3.3
通讯作者：
Habbema, J. Dik F.
中科院分区：
文献类型：
--
作者：
Cao, Wu-Chun;Zhan, Lin;Habbema, J. Dik F.
采用聚合酶链式反应和序列分析相结合的方法，对吉林省中国林区的硬体和啮齿动物体内的自然无浆体吞噬细胞性感染进行了调查。在100只全沟硬蜱中检出4只(4.0%)，在286只森林革螨中检出2只(0.7%)阳性。102只啮齿动物中有9只(8.8%)被感染，25只啮齿动物上寄生的71只过硫杆菌中有2只被感染(2.8%)。从啮齿动物和硬蜱中扩增出的1.442个碱基的16S rRNA基因核苷酸序列与黑龙江省报道的中国的序列完全一致(GenBank登录号：AF205140)，但与其他国家不同。以上标本的柠檬酸合成酶基因部分序列均为同源序列，与已知的吞噬弧菌不同。这些发现增加了吞噬艾美耳球虫生态特征的新信息，并表明该地区存在无浆体病的威胁。
Polymerase chain reaction integrated with sequence analysis was carried out to investigate the natural Anaplasma phagocytophilum infection in ticks and rodents from a forest area of Jilin Province, China. Four (4.0%) of 100 Ixodes persulcatus and 2 (0.7%) of 286 Dermacentor silvarum ticks collected by flagging vegetation were positive. Nine (8.8%) of 102 rodents were infected, as well as 2 (2.8%) of 71 I. persulcatus parasitizing on 25 rodents. The nucleotide sequences of 1.442-bp A. phagocytophilum 16S rRNA gene amplified from rodents and ticks were identical to each other and to that previously reported in Heilongjiang Province of China (GenBank accession no. AF205140), but different from those of other countries. The sequences of 357-bp partial citrate synthase gene from the above specimens were homologic, and varied from known A. phagocytophilum agents. These findings add new information on the ecologic features of A. phagocytophilum and indicate the threat of anaplasmosis in the area.