A human claudin-1-derived peptide inhibits hepatitis C virus entry.
A human claudin-1-derived peptide inhibits hepatitis C virus entry.
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DOI：
10.1002/hep.25685
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发表时间：
2012-08
期刊：
影响因子：
13.5
通讯作者：
Yang, Wei
中科院分区：
文献类型：
--
作者：
Si, Youhui;Liu, Shufeng;Liu, Xiuying;Jacobs, Jana L.;Cheng, Min;Niu, Yuqiang;Jin, Qi;Wang, Tianyi;Yang, Wei
Hepatitis C virus (HCV) entry is a complicated process that requires multiple host factors, such as CD81, scavenger receptor BI, claudin-1 (CLDN1), and occludin. The interaction of virus and cellular entry factors represents a promising target for novel anti-HCV drug development. In this study, we sought to identify peptide inhibitors for HCV entry by screening a library of overlapping peptides covering the four above-mentioned entry factors. An 18–amino acid peptide (designated as CL58) that was derived from the CLDN1 intracellular and first transmembrane region inhibited both de novo and established HCV infection in vitro. Unlike previously reported peptides corresponding to CLDN1 extracellular loops, CL58 did not alter the normal distribution of CLDN1 and was not cytotoxic in vitro at concentrations nearly 100-fold higher than the effective antiviral dose. The inhibitory effect of CL58 appeared to occur at a late step during viral entry, presumably after initial binding. Finally, overexpressed CL58 was able to interact with HCV envelope proteins. We identified a novel CLDN1-derived peptide that inhibits HCV entry at a postbinding step. The findings expand our knowledge of the roles that CLDN1 play in HCV entry and highlight the potential for developing a new class of inhibitors targeting the viral entry process.
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影响因子：
5.4
作者：
Meertens, Laurent;Bertaux, Claire;Dragic, Tatjana
通讯作者：
Dragic, Tatjana
影响因子：
11.4
作者：
Scarselli, E;Ansuini, H;Vitelli, A
通讯作者：
Vitelli, A
影响因子：
13.5
作者：
Krieger, Sophie E.;Zeisel, Mirjam B.;Baumert, Thomas F.
通讯作者：
Baumert, Thomas F.
DOI：
10.1083/jcb.136.2.399
发表时间：
1997-01-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Wong V;Gumbiner BM
通讯作者：
Gumbiner BM
影响因子：
5.4
作者：
Liu, Shufeng;Yang, Wei;Wang, Tianyi
通讯作者：
Wang, Tianyi