Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
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DOI：
10.1073/pnas.0801866105
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发表时间：
2008-07-15
影响因子：
11.1
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
我们提出了一种自下而上的方法来指导组装载细胞的微凝胶，以产生具有可调微结构和复杂性的组织结构。该组装过程由多相液-液系统的趋势驱动，以使表面积和相之间的所得表面自由能最小化。我们证明，形状控制的微凝胶自发组装成预定的几何构型内的多相反应器系统。此外，我们表征的参数，影响组装过程中，如外部能量输入，表面张力，和微凝胶尺寸。最后，我们表明，多组分细胞负载的结构可以通过组装微凝胶积木和进行二次交联反应。这种自下而上的方法用于定向组装载有细胞的微凝胶，提供了一种强大的和高度可扩展的方法来形成仿生3D组织构建体，并打开了一个范例，用于指导组装的介观材料。
We present a bottom-up approach to direct the assembly of cell-laden microgels to generate tissue constructs with tunable microarchitecture and complexity. This assembly process is driven by the tendency of multiphase liquid-liquid systems to minimize the surface area and the resulting surface free energy between the phases. We demonstrate that shape-controlled microgels spontaneously assemble within multiphase reactor systems into predetermined geometric configurations. Furthermore, we characterize the parameters that influence the assembly process, such as external energy input, surface tension, and microgel dimensions. Finally, we show that multicomponent cell-laden constructs could be generated by assembling microgel building blocks and performing a secondary cross-linking reaction. This bottom-up approach for the directed assembly of cell-laden microgels provides a powerful and highly scalable approach to form biomimetic 3D tissue constructs and opens a paradigm for directing the assembly of mesoscale materials.