NUCLEOTIDE-SEQUENCE AND GENOMIC ORGANIZATION OF A TANDEM SATELLITE ARRAY FROM THE ROCK VOLE MICROTUS-CHROTORRHINUS (RODENTIA)
NUCLEOTIDE-SEQUENCE AND GENOMIC ORGANIZATION OF A TANDEM SATELLITE ARRAY FROM THE ROCK VOLE MICROTUS-CHROTORRHINUS (RODENTIA)
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DOI：
10.1007/bf00355723
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发表时间：
1992-01-01
期刊：
影响因子：
2.5
通讯作者：
MODI, WS
中科院分区：
文献类型：
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作者：
MODI, WS
一个串联的卫星阵列（本文命名为MSAT-160）已被分离和表征的啮齿动物田鼠chrotorabus。15个部分或完整单体的序列数据显示重复单元长度为160 bp。这个单位长度显然是来自两个较短的亚基序，一个是四聚体（GAAA），另一个是六聚体（CTTTCT），通过聚合酶滑移和突变。总的来说，这两个基序的完美或不完美变体占该成分的近60%。用14种不同的限制性内切酶消化基因组DNA的Southern印迹分析表明，大多数酶产生经典的A型或B型限制性图谱，而RsaI产生的图谱结合了A型和B型的特征，BamHI似乎在MSAT-160中完全缺乏位点。用三种酶对16个个体的限制性图谱进行检查，未能确定种内变异，而一项相关研究比较了11个物种，并记录了种间差异（Modi，提交）。荧光原位杂交结果表明，该卫星DNA位于几个常染色体的着丝粒和性染色体异染色质上（GenBank登录号M86843）。
A tandem satellite array (herein named MSAT-160) has been isolated and characterized from the rodent Microtus chrotorrhinus. Sequence data from 15 partial or complete monomers revealed a repeat unit length of 160 bp. This unit length was apparently derived from two shorter sub-motifs, one a tetramer (GAAA), the other a hexamer (CTTTCT), through polymerase slippage and mutation. Collectively, perfect or imperfect variants of these two motifs comprise nearly 60% of the component. Southern blot analyses of genomic DNA digested with 14 different restriction endonucleases indicated that most enzymes yielded either classical type A or type B restriction patterns, while RsaI yielded a pattern that combined features of both the A and B types, and BamHI appeared to lack sites altogether in MSAT-160. An examination of restriction patterns from 16 individuals with three enzymes failed to identify intraspecific variation, while a related study compared 11 species and documented interspecific distinctiveness (Modi, submitted). Fluorescence in situ hybridization indicated that the satellite DNA was located at the centromeres of several autosomes and at sex chromosome heterochromatin (GenBank accession No. M86843).