Effects of a Shaker K+ channel peptide and trypsin on a K+ channel in Necturus enterocytes.
Effects of a Shaker K+ channel peptide and trypsin on a K+ channel in Necturus enterocytes.
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Shaker K 通道肽和胰蛋白酶对 Necturus 肠上皮细胞 K 通道的影响。
DOI：
10.1152/ajpcell.1993.265.2.c541
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Schultz,SG
中科院分区：
文献类型：
--
作者：
Mayorga-Wark,O;Costantin,J;Dubinsky,WP;Schultz,SG
我们先前已经证明，由Shaker B K+通道蛋白NH 2-末端的前22个氨基酸组成的合成肽使重构为平面脂质双层的Necturus小肠上皮细胞的基底外侧膜中存在的电压依赖性K+通道失活（Dubinsky等人，Proc.Natl.Chem.2004，28：Acad. Sci. USA 89：1770-1774，1992）。我们现在证明，这种肽相互作用的Necturus通道的内表面，只有当它是在开放或导电配置，这种相互作用是由四乙基铵离子，一个完善的阻断剂，这个和其他K+通道。我们的结论是，这种肽是Necturus K+通道的开孔阻滞剂，因为它似乎是在振荡器B K+通道的情况下。我们进一步证明，胰蛋白酶，这废除了这种肽的能力，以阻止Necturus和振荡器K+通道和抑制自发失活的振荡器K+通道，也损害了Necturus K+通道的电压门。这些发现，以及其他将在配套文件中报告的结果，表明Shaker B K+通道的“失活肽”和Necturus K+通道的电压门之间的结构同源性。
We have previously demonstrated that a synthetic peptide composed of the first 22 amino acids from the NH2-terminus of the Shaker B K+ channel protein deactivates a voltage-dependent K+ channel present in basolateral membrane of Necturus small intestinal epithelial cells reconstituted into planar lipid bilayers (Dubinsky et al. Proc. Natl. Acad. Sci. USA 89: 1770-1774, 1992). We now demonstrate that this peptide interacts with the inner surface of the Necturus channel only when it is in the open or conducting configuration and that this interaction is hindered by tetraethylammonium ion, a well-established blocker of this and other K+ channels. We conclude that this peptide is an open-pore blocker of the Necturus K+ channel as it appears to be in the case of the Shaker B K+ channel. We further demonstrate that trypsin, which abolishes the ability of this peptide to block both the Necturus and the Shaker K+ channels and inhibits spontaneous inactivation of the Shaker K+ channel, also impairs the voltage-gate of the Necturus K+ channel. These findings, and others to be reported in a companion paper, suggest structural homologies between the "inactivation peptide" of the Shaker B K+ channel and the voltage-gate of the Necturus K+ channel.
来自果蝇 Shaker K 通道的肽可抑制 Necturus 肠细胞基底外侧膜中的电压门控 K 通道。
DOI：
10.1073/pnas.89.5.1770
发表时间：
1992
影响因子：
11.1
作者：
Dubinsky,WP;Mayorga-Wark,O;Schultz,SG
通讯作者：
Schultz,SG