Potential misidentification of a new Exiguobacterium sp as Oerskovia xanthineolytica isolated from blood culture
Potential misidentification of a new Exiguobacterium sp as Oerskovia xanthineolytica isolated from blood culture
复制标题
DOI：
10.1080/09674845.2006.11732726
复制
发表时间：
2006-01-01
影响因子：
1.9
通讯作者：
Moore, J. E.
中科院分区：
文献类型：
--
作者：
Kenny, F.;Xu, J.;Moore, J. E.
在Sligo总医院就诊的一名男性患者的血培养呈棒状样革兰氏阳性，通过API Coryne试剂盒（法国bioMerieux）对该菌进行表型鉴定，结果为Oerskovia xanthineolytica（分离物鉴定号：290703/S6645SLIGO）。然而，鉴于获得的表型鉴定相对较差，随后通过聚合酶链反应（PCR）扩增和16S rRNA基因大但部分区域的直接测序，将分离物转发给分子鉴定，如该小组先前所述。1 - 3利用FASTA比对软件（www.fasta）与GenBank数据系统中存储的序列进行比对，得到1024 bp的序列。ebi。ac. uk)，并存入GenBank（登录号：AY360351）。BLAST分析与先前报道的用于解释部分16S rRNA基因序列的标准相结合，4
Blood culture of a male patient attending Sligo General Hospital became positive with a coryneform-like Grampositive rod, and phenotypic identification of the organism by the API Coryne kit (bioMerieux, France) yielded Oerskovia xanthineolytica (isolate identifier: 290703/S6645SLIGO). The isolate grew aerobically on blood agar at 37 C. However, given the relatively poor phenotypic identification obtained, the isolate subsequently was forwarded for molecular identification through polymerase chain reaction (PCR) amplification and direct sequencing of a large but partial region of the 16S rRNA gene, as previously described by this group. 1–3The resulting sequence obtained (1024 bp) was compared with those stored in the GenBank Data system using FASTA alignment software (www. ebi. ac. uk), and was deposited in GenBank (accession number: AY360351). On BLAST analysis in combination with previously reported criteria used for interpretation of partial 16S rRNA gene sequences, 4 the