Tree shrew as a new animal model for the study of lung cancer.
Tree shrew as a new animal model for the study of lung cancer.
复制标题
树鼩作为研究肺癌的新动物模型
DOI：
10.3892/ol.2016.4156
复制
发表时间：
2016-03
期刊：
影响因子：
2.9
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Ye L;He M;Huang Y;Zhao G;Lei Y;Zhou Y;Chen X
动物模型在确定包括肺癌在内的各种癌症的治疗方法中起着关键作用。本研究的目的是利用中国云南地区的树鼩开发一种新的肺癌诱导动物模型。树鼩适合全面模拟人类疾病，因为它们的结构、功能和代谢与人类足够接近。这种动物可能为肺癌研究提供一种新的实验动物模型。在本研究中，80只健康树鼩被分配到实验组和对照组。实验组动物通过气管内滴注接受不同浓度的3 - 甲基胆蒽（3 - MC）和二乙基亚硝胺（DEN）的碘油混悬液，而对照组动物通过气管内滴注接受生理盐水或碘化油溶剂。在第3、5、7、9和11周测量动物体重并进行胸部X射线检查。还对肺组织进行了病理学研究，并检查了所有组中支气管上皮发生的病理变化。实验组动物逐渐体重减轻。空白对照组和溶剂对照组树鼩的存活率分别为100%和80%，而实验组的存活率为0%。对空白对照组和溶剂对照组动物进行的胸部X射线检查结果显示无明显异常，而在实验组观察到灌注部位内有高密度阴影斑。对这些高密度区域进行的病理学研究证实了支气管上皮的变化。在实验组中，我们还检测到支气管上皮不典型增生以及原位癌的明显变化。总之，通过一次性气管内引入3 - MC和DEN的碘油混悬液，成功地在树鼩中诱导出肺癌。
Animal models play a key role in identifying treatments for various types of cancer, including lung cancer. The aim of the present study was to develop a new animal model for lung cancer induction using tree shrews from the Yunnan region in China. Tree shrews are suitable for a full simulation of human disease because their structure, function and metabolism are adequately close to human. This animal may offer a new experimental animal model to be used in the study of lung cancer. In the present study, 80 healthy tree shrews were distributed in experimental and control groups. Animals in the experimental group received different concentrations of iodized oil suspension of 3-methylcholanthrene (3-MC) and diethylnitrosamine (DEN) while animals in the control groups received saline or lipiodol solvent via endotracheal instillation. In the 3rd, 5th, 7th, 9th and 11th weeks the body weights of the animals were measured and chest X-ray examinations were conducted. Pathological studies on the lung tissues were also performed and the pathological changes occurring in bronchial epithelium in all the groups were examined. Animals in the experimental group gradually lost their body weight. For tree shrews in the blank control and solvent control groups the survival rates were 100 and 80%, respectively while the survival rate for the experimental group was 0%. Results from the chest X-ray conducted on animals in the blank control and solvent control groups revealed no obvious abnormalities while in the experimental group high-density shadow spots within the perfusion sites were observed. Pathological studies performed on these high-density areas confirmed changes in the bronchial epithelium. In the experimental groups we also detected bronchial epithelial atypical hyperplasia, and apparent changes in carcinoma in situ. In conclusion, lung cancer was successfully induced in tree shrews by a one-time endotracheal introduction of iodized oil suspension of 3-MC and DEN.