Dynamic Contrast Enhanced Optical Imaging of Capillary Leakage
Dynamic Contrast Enhanced Optical Imaging of Capillary Leakage
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DOI：
10.7785/tcrt.2012.500179
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Clement, O.
中科院分区：
文献类型：
--
作者：
Faye, N.;Fournier, L.;Clement, O.
我们使用纤维共聚焦荧光显微镜系统在体内研究了两种荧光对比剂在三种类型毛细血管中的血管通透性。小鼠注射大分子（白蛋白FITC 68,000道尔顿）或低分子量造影剂（FITC 389道尔顿）后成像。我们研究了肌肉中的连续毛细血管（FITC n = 4，白蛋白FITC n = 6），肠系膜中的有孔毛细血管（FITC n = 8，白蛋白FITC n = 10），以及异种移植肿瘤中的不连续毛细血管（FITC n = 2，白蛋白FITC n = 4）。测量毛细血管和间隙区信号强度（SI），绘制时间增强曲线。建立双室模型确定定量微循环参数。在肠系膜和肌肉毛细血管中观察到两种不同造影剂丸的到达，但在肿瘤毛细血管中没有观察到。低分子量造影剂的间质渗漏几乎是瞬间观察到的，而大分子造影剂则留在血管内。在观察期间，信号强度下降，特别是在肿瘤中。由于模型的不稳定性，两种双室模型均无法获得定量的微循环参数。本研究表明，该纤维荧光成像装置可在体内不同类型的毛细血管中重复性地观察微循环。需要进一步的工作来量化微血管参数。
We studied in vivo the vascular permeability of two fluorescent contrast agents in three types of capillary, using a fibered confocal fluorescence microscopy system. Mice were imaged after injection of a macromolecular (albumin FITC 68,000 daltons) or low-molecular-weight contrast agent (FITC 389 daltons). We studied continuous capillaries in muscles (FITC n = 4, albumin FITC n = 6), fenestrated capillaries in mesenteries (FITC n = 8, albumin FITC n = 10), and discontinuous capillaries in xenografted tumors (FITC n = 2, albumin FITC n = 4). Signal intensity (SI) was measured in capillary and interstitial regions, and time-enhancement curves were drawn. Two-compartment models were constructed to determine quantitative microcirculation parameters. The arrival of the bolus of the two different contrast agents was observed in mesentery and muscle capillaries but not in tumor capillaries. Interstitial leakage of the low-molecular-weight contrast agent was observed almost instantaneously, whereas the macromolecular agent remained within the vessels. Signal intensity declined over the observation period, specifically in the tumor. No quantitative microcirculation parameters could be obtained with either of two bi compartmental models, owing to model instability. This study shows that the microcirculation can be reproducibly observed in different types of capillary in vivo with this fibered fluorescence imaging device. Further work is required to quantify microvascular parameters.