The effect of low and high molecular weight chitosan on the control of gray mold (Botrytis cinerea) on kiwifruit and host response
The effect of low and high molecular weight chitosan on the control of gray mold (Botrytis cinerea) on kiwifruit and host response
复制标题
低分子量和高分子量壳聚糖对猕猴桃灰霉病（Botrytis cinerea）的控制效果和寄主反应
DOI：
10.1016/j.scienta.2018.11.038
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发表时间：
2019-02-27
影响因子：
4.3
通讯作者：
Liu, Yongsheng
中科院分区：
文献类型：
--
作者：
Hua, Chenyan;Li, Yulin;Liu, Yongsheng
研究了低分子量壳聚糖（LMWC，3.4- 51.3kDa）和高分子量壳聚糖（HMWC，136.8- 342.0kDa）对猕猴桃灰霉病的抑制作用。结果表明，低分子量碳和高分子量碳均能显著抑制灰霉病的发生，且低分子量碳的抑制作用大于高分子量碳。LMWC对B孢子萌发和菌丝生长的抑制作用强于HMWC。灰叶LMWC还诱导了更高水平的过氧化氢（H2 O2）的积累，以及更高水平的几种防御相关酶的活性。此外，LMWC比HMWC具有更强的穿透猕猴桃表皮组织细胞壁的能力。虽然几丁质激发子受体激酶1（CERK 1），有丝分裂原活化蛋白激酶（MPK 3），PR 1和PR 5的表达显着诱导猕猴桃由LMWC和HMWC，CERK 1和PR 5的诱导水平更高的LMWC诱导。总的来说，结果表明LMWC比HMWC更有效地抑制猕猴桃的灰霉病，这很可能是由于LMWC更容易穿透猕猴桃的表皮细胞壁并激活更大的防御反应的能力。
The inhibitory effect of low (LMWC, 3.4-51.3 kDa) and high molecular weight chitosan (HMWC, 136.8-342.0 kDa) on gray mold (Botrytis cinerea) of kiwifruit was evaluated. Results indicated that both LMWC and HMWC significantly inhibited the development of gray mold, with LMWC having a greater inhibitory effect than HMWC. LMWC, was more effective than HMWC in inhibiting spore germination and mycelial growth of B. cinerea. LMWC also induced the accumulation of higher levels of hydrogen peroxide (H2O2), and greater level of activity of several defense-related enzymes. Moreover, LMWC had a greater ability than HMWC to penetrate the cell walls in kiwifruit epidermal peel tissue. While the expression of chitin elicitor receptor kinase 1 (CERK1), mitogen-activated protein kinase (MPK3), PR1, and PR5 were significantly induced in kiwifruit by both LMWC and HMWC, the level of induction of CERK1 and PR5 was more highly induced by LMWC. Collectively, the results indicate that LMWC is more effective than HMWC in inhibiting gray mold of kiwifruit, most likely due to the ability of LMWC to more readily penetrate the epidermal cell walls of kiwifruit and activate a greater defense response.