Isolation and characterization of Acinetobacter sp. ND12 capable of degrading nicotine
Isolation and characterization of Acinetobacter sp. ND12 capable of degrading nicotine
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DOI：
10.5897/ajmr11.210
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发表时间：
2011-06
影响因子：
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通讯作者：
Hongjuan Li;Y. Duan;Guanghui Ma;Liping Lei;Ke-Qin Zhang;Jinkui Yang
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文献类型：
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作者：
Hongjuan Li;Y. Duan;Guanghui Ma;Liping Lei;Ke-Qin Zhang;Jinkui Yang
从烟草种植土壤中分离到一株菌株ND12，根据其形态、生理和16S rRNA基因序列鉴定为不动杆菌属（acinetobacter sp.）。该分离物以烟碱为唯一碳氮来源，在28℃、pH 6.0条件下，14 h内完全降解烟碱1.0 g/L。菌株ND12生长的最佳烟碱浓度为2.5 g/L。菌株ND12在较宽的pH范围内生长，在酸性环境（pH 4.0）下表现出较高的尼古丁降解活性。经11 h处理后，动杆菌ND12的静息细胞可分解烟叶上部90%的烟碱。利用高效液相色谱法分析了ND12的烟碱代谢产物，并与HJ2-3节杆菌和Nic22假单胞菌进行了比较。研究结果表明，菌株ND12可能有助于降低尼古丁污染环境和烟草废物中的尼古丁浓度。关键词：尼古丁，生物降解，不动杆菌sp. ND12，静息细胞，烟叶
A bacterium strain ND12, was isolated from the tobacco plantation soil and identified asAcinetobacter sp. based on its morphology, physiology, and 16S rRNA gene sequence. The isolate could utilize nicotine as the sole source of carbon and nitrogen, and completely degrade 1.0 g/L nicotine within 14 h at 28°C and pH 6.0. The optimum nicotine concentration for the growth of strain ND12 was 2.5 g/L. Strain ND12 grew at a broader pH range than other reported nicotine-degrading bacteria and showed higher nicotine-degrading activity than those bacteria at acidic environment (pH 4.0). The resting cells ofAcinetobacter sp. ND12 could decompose 90% of the nicotine in upper tobacco leaves after treating for 11 h. Moreover, the nicotine metabolites of strain ND12 were analyzed byhigh performance liquid chromatography (HPLC) and compared with Arthrobacter sp. HJ2-3 and Pseudomonas sp. Nic22. Our results suggested that strain ND12 could be useful for reducing nicotine concentration in nicotine-polluted environments and tobacco wastes. Key words: Nicotine, biodegradation, Acinetobacter sp. ND12, resting cells, tobacco leaves.