Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats
Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats
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DOI：
10.1016/j.jss.2006.06.034
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发表时间：
2007-03-01
影响因子：
2.2
通讯作者：
Kamiyama, Yasuo
中科院分区：
文献类型：
--
作者：
Qiu, Zeyu;Kwon, A-Hon;Kamiyama, Yasuo
背景纤连蛋白已被证明有助于伤口愈合。糖尿病伤口愈合受损的特征在于伤口部位血浆纤连蛋白（pFn）的减少。本研究探讨局部应用pFn是否可以改善糖尿病大鼠受损的伤口愈合。在链脲佐菌素（STZ）诱导的糖尿病大鼠背部造成全层皮肤创伤。立即将人pFn引入伤口床，而接受人血清白蛋白或生理盐水的伤口用作对照。使用公认的伤口愈合参数监测伤口闭合：组织学检查上皮形成、血管形成、胶原沉积和成纤维细胞迁移。转化生长因子（TGF）-β 1通过免疫化学测定。还评估了伤口皮肤中的羟脯氨酸水平。局部应用pFn显著加速伤口闭合。此外，pFn处理的伤口显示增加的成纤维细胞血管化、胶原再生和上皮形成。pFn处理的伤口中表达TGF-β 1和羟脯氨酸水平的浸润成纤维细胞的数量显著高于对照组。pFn可以改善糖尿病伤口的受损愈合，并且这种作用可能涉及成纤维细胞活性的增加和TGF-β 1释放的增加。(c)2007年爱思唯尔公司All rights reserved.
Background. Fibronectin has been shown to assist in wound healing. Impaired wound healing in diabetes mellitus is characterized by a reduction in plasma fibronectin (pFn) at the wound site. This study investigated whether topical application of pFn could improve the impaired wound healing in diabetic rats.Materials and methods. Full-thickness skin wounds were created on the backs of streptozotocin (STZ)induced diabetic rats. Immediately, human pFn was introduced into the wound bed, while wounds receiving human serum albumin or normal saline were used as controls. Wound closure was monitored using well-recognized wound-healing parameters: epithelialization, vascularization, collagen deposition, and migration of fibroblasts were examined histologically. Transforming growth factor (TGF)-beta 1 was measured by immunochemistry. Hydroxyproline levels also were assessed in the wound skin.Results. Wound closure was significantly accelerated by local application of pFn. Furthermore, pFn-treated wounds showed increased fibroblast vascularization, collagen regeneration, and epithelialization. The numbers of infiltrating fibroblasts expressing TGF-beta 1 and hydroxyproline levels in pFn-treated wounds were significantly higher than those in the controls.Conclusions. pFn can improve the impaired healing of diabetic wounds and this effect might involve an increase in the activity of fibroblasts and increased release of TGF-beta 1. (c) 2007 Elsevier Inc. All rights reserved.