Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II
Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II
复制标题
DOI：
10.1242/dev.050120
复制
发表时间：
2010-09-15
期刊：
影响因子：
4.6
通讯作者：
Sawamoto, Kazunobu
中科院分区：
文献类型：
--
作者：
Hirota, Yuki;Meunier, Alice;Sawamoto, Kazunobu
游动的纤毛在上皮组织上产生持续的液体流动，从而影响各种生理过程。纤毛细胞的这种功能依赖于纤毛的平面极性和它们的基体朝向流体流动的下游方向。最近，另一种类型的基底体平面极性被报道，其特征是基底体在单个细胞中的前部定位，位于脑室表面的多纤毛的室管膜细胞中。然而，人们对这种极性建立的细胞和分子机制知之甚少。在这里，我们在小鼠身上报道，在分化过程中，基底小体在顶端细胞膜中移动，积聚在室管膜细胞的前部。在新生儿脑中，平面细胞极性信号通路影响基础体定向，但不影响它们的前向迁移。此外，我们通过药理学和遗传学研究表明，非肌肉肌球蛋白II是这种基本体分布的关键调节因子。这项研究表明，基底的取向和分布是通过不同的机制发生的。
Motile cilia generate constant fluid flow over epithelial tissue, and thereby influence diverse physiological processes. Such functions of ciliated cells depend on the planar polarity of the cilia and on their basal bodies being oriented in the downstream direction of fluid flow. Recently, another type of basal body planar polarity, characterized by the anterior localization of the basal bodies in individual cells, was reported in the multiciliated ependymal cells that line the surface of brain ventricles. However, little is known about the cellular and molecular mechanisms by which this polarity is established. Here, we report in mice that basal bodies move in the apical cell membrane during differentiation to accumulate in the anterior region of ependymal cells. The planar cell polarity signaling pathway influences basal body orientation, but not their anterior migration, in the neonatal brain. Moreover, we show by pharmacological and genetic studies that non-muscle myosin II is a key regulator of this distribution of basal bodies. This study demonstrates that the orientation and distribution of basal bodies occur by distinct mechanisms.