Reconstituting Nuclear and Chromosome Dynamics Using Xenopus Extracts.
Reconstituting Nuclear and Chromosome Dynamics Using Xenopus Extracts.
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DOI：
10.1101/pdb.top097105
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发表时间：
2019-03-01
影响因子：
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通讯作者：
Rankin, Susannah
中科院分区：
文献类型：
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作者：
Rankin, Susannah
从青蛙卵中提取的提取物，特别是非洲爪蟾，为几十年来核和染色体动力学的开创性研究提供了关键材料。它们对这些类型的分析的有用性在于几个重要的特征：储存的核成分，内源性DNA的缺乏，以及完整和功能正常的信号网络。这些因素允许对染色体生物学的许多方面进行详细的分子分析，包括DNA复制，检查点信号传导，表观遗传控制以及染色体凝聚，凝聚和分离。本文详细介绍了非洲爪蟾卵提取物的制备及其在染色体和染色质研究中的应用。
Extracts prepared from the eggs of frogs, particularly Xenopus species, have provided critical material for seminal studies of nuclear and chromosome dynamics over several decades. Their usefulness for these types of analyses lies in several important characteristics: stockpiled nuclear components, absence of endogenous DNA, and intact and functioning signaling networks. These factors have allowed detailed molecular analyses of many aspects of chromosome biology, including DNA replication, checkpoint signaling, epigenetic control, and chromosome condensation, cohesion, and segregation. In this introduction, the preparation and application of Xenopus egg extracts for the study of chromosomes and chromatin are described in detail.