EIN3 and SOS2 synergistically modulate plant salt tolerance.
EIN3 and SOS2 synergistically modulate plant salt tolerance.
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EIN3和SOS2协同调节植物耐盐性
DOI：
10.1038/srep44637
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发表时间：
2017-03-16
影响因子：
4.6
通讯作者：
Huang R
中科院分区：
文献类型：
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作者：
Quan R;Wang J;Yang D;Zhang H;Zhang Z;Huang R
乙烯生物合成和乙烯信号通路通过激活与ROS和Na+/K+稳态相关的下游靶基因的表达来调节植物的耐盐性。盐过度敏感（SOS）通路调控盐胁迫下拟南芥Na+/K+稳态。然而，这两种途径之间的联系尚不清楚。通过遗传筛选，我们在ein3-1背景下鉴定出两个sos2等位基因为盐敏感突变体。乙烯不敏感2 （EIN2）和EIN3均未改变sos1、SOS2、sos3和sos3样钙结合蛋白8 （scasp8）等sos基因的表达模式，但SOS2激活了EIN3的一个靶基因——乙烯盐诱导型erf1 （ESE1）的表达。此外，丝氨酸/苏氨酸蛋白激酶SOS2在体外主要在S325位点磷酸化ein3，在S35、T42和S606位点弱磷酸化。在瞬时GUS实验中，EIN3 S325A突变降低了其转录激活活性onese1启动子：GUS，并损害了其拯救EIN3 -1盐过敏的能力。此外，SOS2还激活了盐胁迫下盐反应性ESE1靶基因的表达。因此，EIN3-SOS2可能在拟南芥盐响应中连接乙烯信号通路和SOS信号通路。
Ethylene biosynthesis and the ethylene signaling pathway regulate plant salt tolerance by activating the expression of downstream target genes such as those related to ROS and Na+/K+homeostasis. The Salt Overly Sensitive (SOS) pathway regulates Na+/K+homeostasis inArabidopsisunder salt stress. However, the connection between these two pathways is unclear. Through genetic screening, we identified twosos2alleles as salt sensitive mutants in theein3-1background. Neither Ethylene-Insensitive 2 (EIN2) nor EIN3 changed the expression patterns ofSOSgenes includingSOS1, SOS2, SOS3andSOS3-like Calcium Binding Protein 8 (SCaBP8), but SOS2 activated the expression of one target gene of EIN3,Ethylene and Salt-inducible ERF 1 (ESE1). Moreover, Ser/Thr protein kinase SOS2 phosphorylated EIN3in vitromainly at the S325 site and weakly at the S35, T42 and S606 sites. EIN3 S325A mutation reduced its transcriptional activating activity onESE1promoter:GUS in a transient GUS assay, and impaired its ability to rescueein3-1salt hypersensitivity. Furthermore, SOS2 activated salt-responsive ESE1 target gene expression under salt stress. Therefore, EIN3-SOS2 might link the ethylene signaling pathway and the SOS pathway inArabidopsissalt responses.