MiR-26a performs converse roles in proliferation and metastasis of different gastric cancer cells via regulating of PTEN expression
MiR-26a performs converse roles in proliferation and metastasis of different gastric cancer cells via regulating of PTEN expression
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MiR-26a通过调节PTEN表达在不同胃癌细胞的增殖和转移中发挥相反作用
DOI：
10.1016/j.prp.2017.01.026
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Tan, Sheng
中科院分区：
文献类型：
--
作者：
Ding, Keshuo;Wu, Zhengsheng;Tan, Sheng
胃癌是全球癌症相关死亡的第二大原因。胃癌中确切的分子通路需要进一步研究。在此我们指出，miR - 26a在不同的胃癌细胞中对致癌性起着相反的作用。在胃癌细胞MKN - 28中，miR - 26a促进细胞增殖、迁移和侵袭。然而，在胃癌细胞AGS中，miR - 26a减少细胞增殖和转移。PTEN被确定为miR - 26a的直接靶标。在MKN - 28细胞中，miR - 26a通过3' - UTR抑制PTEN，并且PTEN介导miR - 26a促进致癌性，包括细胞增殖和转移。另一方面，在AGS细胞中，miR - 26a增强PTEN的表达，并且PTEN介导miR - 26a降低致癌性。AGS细胞中的机制可能是miR - 26a对PTEN的间接调控克服了直接靶向调控。像MKN - 28细胞这样的模式与miR - 26a高水平且PTEN低水平的患者以及miR - 26a低水平且PTEN高水平的患者相符，这些患者总体生存率（OS）较低；像AGS细胞这样的模式与miR - 26a和PTEN均高水平以及miR - 26a和PTEN均低水平的患者相符，这些患者总体生存率较高。这些发现将有助于更好地理解miR - 26a的功能和机制，miR - 26a和PTEN是胃癌患者潜在的联合生物标志物。（C）2017爱思唯尔集团。保留所有权利。
Gastric cancer is the second leading cause of cancer-related death in the world. The exact molecular pathways in gastric cancer need for further study. We herein indicated miR-26a performed converse roles on oncogenicity in different gastric cancer cells. In gastric cancer cells MKN-28, miR-26a promoted cell proliferation, migration and invasion. However, in gastric cancer cells AGS, miR-26a reduced cell proliferation and metastasis. PTEN was identified as a direct target of miR-26a. In MKN-28 cells, PTEN was suppressed by miR-26a through 3'-UTR, and PTEN mediated miR-26a promoting oncogenicity including cell proliferation and metastasis. On the other hand, in AGS cells, the expression of PTEN was enhanced by miR-26a, and PTEN mediated miR-26a reducing oncogenicity. The mechanism in AGS cells may be the indirect regulation of PTEN by miR-26a overcame the direct targeting regulation. The model like MKN-28 cells was concordant with patients with a high level of miR-26a and a low level of PTEN and patients with a low level of miR-26a and a high level of PTEN which showed lower overall survival (OS); the model like AGS cells was concordant with patients with both high level of miR-26a and PTEN and both low level of miR-26a and PTEN which showed higher OS. These findings will facilitate a better understanding of the functions and mechanisms about miR-26a, miR-26a and PTEN are potential combined biomarkers in patients with gastric cancer. (C) 2017 Elsevier GmbH. All rights reserved.