Host/nonhost status and genetics of resistance in barley against three pathotypes of Magnaporthe blast fungi
Host/nonhost status and genetics of resistance in barley against three pathotypes of Magnaporthe blast fungi
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DOI：
10.1007/s10681-019-2436-z
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发表时间：
2019-07-01
期刊：
影响因子：
1.9
通讯作者：
Niks, R. E.
中科院分区：
文献类型：
--
作者：
Aghnoum, R.;Bvindi, C.;Niks, R. E.
稻瘟病菌（Magnaporthe grisea）是由稻瘟病菌（Magnaporthe grisea）和稻瘟病菌（Magnaporthe grisea）复合体引起的一种禾本科植物病害，广泛存在于水稻、小麦和大麦等野生和栽培禾本科植物中。我们接种了一个收集的栽培（大麦亚种。vulgare L.）野生的（ssp。spontaneum）大麦种质与M.稻瘟病菌致病型（MoO）、小麦致病型（MoT）和狼尾草致病型（MsP），以定量分析大麦的寄主地位，并鉴定大麦的抗瘟性来源。与小麦不同，大麦基因库中有丰富的抗稻瘟病菌的完全和部分抗性来源。栽培大麦出现非寄主MsP，而野生大麦表现出一定程度的易感性。所有153份供试水稻材料均对MoT分离物具有抗性，表明水稻对该致病型无寄主性。用M的MoO和/或MoT分离物接种L94/瓦达和瓦达/SusPtrit RIL作图群体。在两个群体中，均发现了一个位于7 H短臂上的抗MoT菌株PY 67.1的大效QTL，命名为Rmoq 1。L94对MoO分离物的抗性是由于位于5 H的不同QTL。西欧大麦品种的关联作图也表明大多数QTL是致病型特异的。在双亲和关联定位研究中发现的6个抗稻瘟病基因与抗白粉病基因Mlt、Mla 6、Mlg、mlo、Mlj和Mlhb的图谱位置一致。我们的QTL和关联作图分析不支持mlo抗性基因与对M.在文献中报道的。
Blast disease, caused by the Magnaporthe oryzae/grisea species complex, occurs in a wide range of wild and cultivated gramineous plant species including rice, wheat and barley. We inoculated a collection of cultivated (Hordeum vulgare ssp. vulgare L.) and wild (ssp. spontaneum) barley accessions with M. oryzae Oryza pathotype (MoO), Triticum pathotype (MoT) and Pennisetum pathotype (MsP) to quantify the host status of barley, and to identify sources of blast resistance. Unlike wheat, the barley gene pool is rich with sources of complete and partial resistance against Magnaporthe. Cultivated barley appeared a nonhost to MsP, whereas wild barley showed some degree of susceptibility. All 153 tested rice accessions were resistant to the MoT isolate, suggesting that rice is nonhost to this pathotype. Inoculation of L94/Vada and Vada/SusPtrit RIL mapping populations with MoO and/or MoT isolates of M. oryzae indicated one large effect QTL, designated as Rmoq1, on the short arm of chromosome 7H against the MoT isolate PY 67.1 in both populations. Resistance in L94 to the MoO isolate was due to a different QTL, located at 5H. An association mapping panel of West European barley cultivars also suggested that most QTLs were pathotype specific. Six blast resistance genes found in the biparental and association mapping studies coincided with map positions of powdery mildew resistance genes viz. Mlt, Mla6, Mlg, mlo, Mlj, and Mlhb genes. Our QTL and association mapping analyses do not support the association of the mlo resistance gene with enhanced susceptibility to M. oryzae as reported in literature.