Long-range interaction between the P2.1 and P9.1 peripheral domains of the tetrahymena ribozyme
Long-range interaction between the P2.1 and P9.1 peripheral domains of the tetrahymena ribozyme
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DOI：
10.1093/nar/25.9.1761
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发表时间：
1997-05-01
影响因子：
14.9
通讯作者：
Inoue, T
中科院分区：
文献类型：
--
作者：
Ikawa, Y;Ohta, H;Inoue, T
四膜虫核酶具有称为P9.1和P9.2的外周结构域。它们在催化反应的机理中不是必需的，但有助于提高核酶的催化活性。据推测，P9.1能够与另一个外周结构域P2.1形成沃森-克里克碱基配对。我们报告存在的远程碱基配对之间的环区域的这两个域，并表明这种相互作用显然起着增强核酶的催化活性的作用。
The Tetrahymena ribozyme possesses peripheral domains, termed P9.1 and P9.2. They are nonessential in the mechanism of the catalytic reaction but contribute to enhance the catalytic activity of the ribozyme. It has been postulated that P9.1 is capable of forming Watson-Crick base pairings with another peripheral domain, P2.1. We report hem the existence of long-range base pairings between the loop regions of these two domains and show that this interaction apparently plays a role in enhancing the catalytic activity of the ribozyme.