Conventional and immunoelectron microscopy of mitochondria.
Conventional and immunoelectron microscopy of mitochondria.
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DOI：
10.1007/978-1-59745-365-3_33
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
McCaffery, J Michael
中科院分区：
文献类型：
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作者：
Perkins, Edward M;McCaffery, J Michael
电子显微镜是研究真核细胞亚细胞结构和功能的重要工具。它提供了有价值的信息组织的高尔基复合体;极化分布的蛋白质质膜;和基本的见解的基本结构和功能的线粒体开始与第一个EM观察克劳德和Fullam在1944年孤立的线粒体。本卷最重要的是免疫电子显微镜（IEM）在线粒体动力学研究中所做的贡献，并证明了酵母中关键线粒体蛋白的定位，包括但不限于Dnm 1 p，Fiz 1 p和Mgm 1 p。本章并不打算对所有EM和IEM方法进行全面的回顾，因为已经有许多关于这些主题的优秀书籍和评论。相反，本章提供了详细的协议，传统的EM和IEM方法成功地利用在我们的中心，在酵母和哺乳动物细胞中的线粒体的检查和分析。
Electron microscopy (EM) has been a central tool in delineating the subcellular organization and function of the eukaryotic cell. It has provided valuable information on the organization of the Golgi complex; the polarized distribution of proteins on the plasma membrane; and fundamental insights into the essential structure and function of mitochondria beginning with the first EM observations of Claude and Fullam on isolated mitochondria in 1944. Most significant for this volume is the contribution immunoelectron microscopy (IEM) has made in the study of mitochondrial dynamics and in demonstrating the localizations of key mitochondrial proteins in yeast, including, though not limited to, Dnm1p, Fiz1p, and Mgm1p. This chapter is not intended to provide a comprehensive review of all EM and IEM methods as there are a number of excellent books and reviews already available on these topics. Rather, this chapter provides detailed protocols of conventional EM and IEM methods successfully utilized in our center for the examination and analysis of mitochondria in yeast and mammalian cells.