Fibroblast stimulation in schistosomiasis. I. Stimulation in vitro of fibroblasts by soluble products of egg granulomas.
Fibroblast stimulation in schistosomiasis. I. Stimulation in vitro of fibroblasts by soluble products of egg granulomas.
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血吸虫病中的成纤维细胞刺激。
DOI：
10.1093/infdis/144.3.254
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
L. Wahl
中科院分区：
文献类型：
--
作者：
D. Wyler;S. Wahl;A. Cheever;L. Wahl
为了进一步了解血吸虫病肝纤维化的发病机制，在体外模型中研究了虫卵肉芽肿与成纤维细胞之间的相互作用。从感染曼氏血吸虫或日本血吸虫的小鼠肝脏中分离的虫卵肉芽肿在体外培养，释放出一种不可透析的物质，刺激静息真皮成纤维细胞增殖。成纤维细胞刺激物质的释放在与肉芽肿孵育的第一个48小时期间保持相对恒定，在此期间肉芽肿在体外保持代谢活性。肉芽肿上清液中的可透析分子在体外干扰成纤维细胞摄取[3H]胸苷，但不抑制细胞分裂。肉芽肿上清液还刺激成纤维细胞分泌前列腺素E2，并引起细胞内环磷酸腺苷水平升高。
To gain further understanding of the pathogenesis of hepatic fibrosis in schistosomiasis, the interaction between egg granulomas and fibroblasts was investigated in an in vitro model. Egg granulomas isolated from livers of mice infected with Schistosoma mansoni or Schistosoma japonicum and cultured in vitro released a nondialyzable substance which stimulated proliferation in resting dermal fibroblasts. The release of the fibroblast-stimulating substance remained relatively constant during the first 48 hr of incubation with granulomas, during which time granulomas remained metabolically active in vitro. A dialyzable molecule(s) in granulomas supernatants interfered with fibroblast uptake of [3H]thymidine in vitro but did not inhibit cellular division. Granulomas supernatants also stimulated fibroblast secretion of prostaglandin E2 and caused an elevation in intracellular levels of cyclic adenosine monophosphate.