The Influence of Fruit Load on the Tomato Pericarp Metabolome in a Solanum chmielewskii Introgression Line Population
The Influence of Fruit Load on the Tomato Pericarp Metabolome in a Solanum chmielewskii Introgression Line Population
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DOI：
10.1104/pp.110.163030
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发表时间：
2010-11-01
期刊：
影响因子：
7.4
通讯作者：
Fernie, Alisdair R.
中科院分区：
文献类型：
--
作者：
Do, Phuc Thi;Prudent, Marion;Fernie, Alisdair R.
最近利用番茄与野生种茄子杂交产生的番茄种间渐渗系证明，光合作用分配效率对番茄果皮的代谢组成有相当大的影响。为了进一步评估源-库相互作用的影响，用气相色谱-质谱法测定了不同种群的代谢物组成。为此，我们利用番茄与野生种茄子种间杂交产生的23个导入系，在高(未修剪)和低(修剪成一个果实)果实的条件下进行杂交。按照这一策略，我们能够比较在两种果实负荷下栽培的植物果实的代谢物组成，以及在导入系群体中初级代谢的网络行为。研究表明，虽然在高果量栽培条件下观察到的代谢数量性状基因座数量(240个)比在低果量栽培条件下(128)多，但在低果量栽培条件下，代谢产物水平的相关性更高。最后，对3号基因型果实负荷互作分析表明，发育和栽培对果实组成的影响大于基因型。与以前记录的这些品系子集的转录谱进行比较，发现代谢物水平的变化与与其新陈代谢相关的基因水平的变化没有相关性。这些发现是在我们目前对番茄代谢源-库相互作用的遗传和环境影响的理解的背景下讨论的，特别是对果实氨基酸含量的理解。
It has been recently demonstrated, utilizing interspecific introgression lines of tomato, generated from the cross between Solanum lycopersicum and the wild species Solanum pennellii, that the efficiency of photosynthate partitioning exerts a considerable influence on the metabolic composition of tomato fruit pericarp. In order to further evaluate the influence of source-sink interaction, metabolite composition was determined by gas chromatography-mass spectrometry in a different population. For this purpose, we used 23 introgression lines resulting from an interspecific cross between S. lycopersicum and the wild species Solanum chmielewskii under high (unpruned trusses) and low (trusses pruned to one fruit) fruit load conditions. Following this strategy, we were able to contrast the metabolite composition of fruits from plants cultivated at both fruit loads as well as to compare the network behavior of primary metabolism in the introgression line population. The study revealed that while a greater number of metabolic quantitative trait loci were observed under high fruit load (240) than under low fruit load (128) cultivations, the levels of metabolites were more highly correlated under low fruit load cultivation. Finally, an analysis of genotype 3 fruit load interactions indicated a greater influence of development and cultivation than genotype on fruit composition. Comparison with previously documented transcript profiles from a subset of these lines revealed that changes in metabolite levels did not correlate with changes in the levels of genes associated with their metabolism. These findings are discussed in the context of our current understanding of the genetic and environmental influence on metabolic source-sink interactions in tomato, with particular emphasis given to fruit amino acid content.