The type VI secretion system 2 of Vibrio parahaemolyticus is regulated by QsvR
The type VI secretion system 2 of Vibrio parahaemolyticus is regulated by QsvR
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副溶血弧菌VI型分泌系统2受QsvR调控
DOI：
10.1016/j.micpath.2020.104579
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发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Zhang, Yiquan
中科院分区：
文献类型：
--
作者：
Qiu, Yue;Hu, Lingfei;Zhang, Yiquan
副溶血性弧菌的VI型分泌系统2(T6SS2)基因座由VPA1027-1024、VPA1043-1028和VPA1044-1046三个操纵子组成。QsvR是副溶血性弧菌的毒力调节因子。本研究通过引物延伸、实时定量聚合酶链式反应、LacZ融合、凝胶迁移率改变分析和DNase I足迹技术研究了QsvR对VPA1027、VPA1043和VPA1044的调控作用。结果表明，QsvR与这三个操纵子的启动子-近端DNA区域结合，激活它们的转录。T6SS2对副溶血弧菌的黏附起主要作用，qsvR缺失显著降低了副溶血弧菌对HeLa细胞的黏附。因此，QsvR不仅是T6SS2基因转录的正调控因子，也是副溶血性弧菌与宿主细胞黏附的介质。
The type VI secretion system 2 (T6SS2) gene locus of Vibrio parahaemolyticus is comprised of three operons, VPA1027-1024, VPA1043-1028, and VPA1044-1046. QsvR is a virulence regulator of V. parahaemolyticus. In this study, the regulation of VPA1027, VPA1043 and VPA1044 by QsvR was investigated by primer extension, quantitative real-time PCR, LacZ fusion, electrophoretic mobility shift assay and DNase I footprinting. The results demonstrated that QsvR binds to the promoter-proximal DNA regions of each of these three operons, activating their transcription. T6SS2 was shown to predominately contribute to V. parahaemolyticus adhesion, with qsvR deletion significantly decreasing V. parahaemolyticus adhesion to HeLa cells. Thus, QsvR is not only a positive regulator of T6SS2 gene transcription but also a mediator of V. parahaemolyticus adhesion to host cells.