NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes
NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes
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DOI：
10.1038/nmeth.1740
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发表时间：
2011-12-01
期刊：
影响因子：
48
通讯作者：
Stanley, Edouard G.
中科院分区：
文献类型：
--
作者：
Elliott, David A.;Braam, Stefan R.;Stanley, Edouard G.
Nkx2-5在整个生命过程中在心脏中表达。我们针对人类胚胎干细胞(HESCs)的NKX2-5基因定位了EGFP序列；NKX2-5(EGFP/w)hESCs有助于心脏分化的量化、hESC来源的承诺心祖细胞(hESC-CPC)和心肌细胞(hESC-CMS)的纯化以及分化方案的标准化。我们使用NKX2-5 EGFP(+)细胞来鉴定VCAM1和Sirpa是在心脏谱系中表达的细胞表面标志。
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.