A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination.
A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination.
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单覆盖和多覆盖宏基因组分箱的比较揭示了广泛的隐藏污染。
DOI：
10.1038/s41592-023-01934-8
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发表时间：
2023
期刊：
影响因子：
48
通讯作者：
Mattock J
中科院分区：
文献类型：
--
作者：
Mattock J
元基因组组合使未培养微生物的研究发生了革命性的变化。在这里，我们在同一组样本上比较了单覆盖和多覆盖入库，并证明了多覆盖入库比单覆盖入库产生了更好的结果，并识别了其他方法所缺少的污染物重叠群和嵌合体入库。虽然资源昂贵，但多覆盖入库是一种更好的方法，应该始终比单覆盖入库更好地执行。
Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.