Investigation into the antibacterial activity of silver, zinc oxide and copper oxide nanoparticles against poultry-relevant isolates of Salmonella and Campylobacter
Investigation into the antibacterial activity of silver, zinc oxide and copper oxide nanoparticles against poultry-relevant isolates of Salmonella and Campylobacter
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DOI：
10.1016/j.foodcont.2018.05.008
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发表时间：
2018-10-01
期刊：
影响因子：
6
通讯作者：
King, Thea
中科院分区：
文献类型：
--
作者：
Duffy, Lesley L.;Osmond-McLeod, Megan J.;King, Thea
纳米技术目前正在为农业、饲料和食品部门的广泛创新技术的发展做出重大贡献。迄今为止，很少有工作已经完成，以调查抗微生物纳米颗粒对家禽业最关注的病原体的功效。这项研究是第一个报道CuO纳米颗粒对沙门氏菌的有效性和Ag和CuO纳米颗粒对弯曲杆菌的有效性。本研究的目的是评估银（Ag）、氧化锌（ZnO）和氧化铜（CuO）纳米颗粒对分离自家禽的沙门氏菌和弯曲杆菌的体外活性。采用微量肉汤稀释法测定其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。所有弯曲杆菌菌株的MIC为Au >= CuO >= ZnO纳米颗粒的顺序。Ag纳米颗粒对沙门氏菌最有效。沙门氏菌菌株的生长动力学也在暴露于液体介质中的纳米颗粒期间进行了评估。生长取决于NP浓度，菌株之间存在一些差异。虽然这些特定尺寸的纳米颗粒对纯细菌培养物有效，但纳米颗粒的抗微生物有效性应在工业相关条件下进一步检查。(C)2018作者由Elsevier Ltd.发布。这是CC BY-NC-ND许可下的开放获取文章。
Nanotechnology is currently contributing substantially to the development of a broad range of innovative technologies in the agricultural, feed and food sector. To date, very little work has been done to investigate the efficacy of antimicrobial nanoparticles against the pathogens of highest concern to the poultry industry. This study is the first to report on the effectiveness of CuO nanoparticles against Salmonella and on the effectiveness of Ag and CuO nanoparticles against Campylobacter. The aim of this study was to assess the in vitro activity of silver (Ag), zinc oxide (ZnO) and copper oxide (CuO) nanoparticles against Salmonella and Campylobacter isolated from poultry. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a broth microdilution method in 96 well plates. The MIC for all Campylobacter strains was in the order of Au >= CuO >= ZnO nanoparticles. Ag nanoparticles were the most effective against Salmonella. The growth kinetics of the Salmonella strains were also assessed during exposure to nanoparticles in liquid media. Growth was dependant on NP concentration with some differences noted between strains. While these specific size nanoparticles are effective against pure cultures of bacteria the antimicrobial effectiveness of the nanoparticles should be further examined under industry-relevant conditions. (C) 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license.