DEVELOPMENT OF EXPRESSION VECTORS FOR TRANSGENIC FISH
DEVELOPMENT OF EXPRESSION VECTORS FOR TRANSGENIC FISH
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DOI：
10.1038/nbt1290-1268
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发表时间：
1990-12-01
期刊：
影响因子：
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通讯作者：
HACKETT, PB
中科院分区：
文献类型：
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作者：
LIU, ZJ;MOAV, B;HACKETT, PB
鱼类的遗传改变对于水生生物技术以及研究脊椎动物发育过程中发生的分子相互作用是重要的。许多鱼类的卵数量多，体积大，透明，外部受精，使它们非常适合遗传操作，特别是生产转基因动物。鱼类基因工程需要合适的表达载体。因此，我们开发了两个鱼类表达载体，FV-1和FV-2，它们含有鲤鱼β-肌动蛋白基因的近端启动子和增强子调控元件以及来自鲑鱼生长激素基因的多聚腺苷酸化信号。在显微注射的鱼卵和组织培养的鱼和哺乳动物细胞中测试了两种鱼表达载体。这两个“全鱼”表达载体应可用于鱼类的基因工程，并已用于转基因鱼的生长促进基因。
Genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. The numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of trans-genic animals. Genetic engineering offish requires suitable expression vectors. Accordingly, we developed two fish expression vectors, FV-1 and FV-2, which contain the proximal promoter and enhancer regulatory elements of the carp β-actin gene and the polyadenylation signal from the salmon growth hormone gene. The two fish expression vectors were tested in microinjected fish eggs and in tissue cultured fish and mammalian cells. These two “all-fish” expression vectors should be useful for genetic engineering of fish and have been used with growth-enhancing genes in transgenic fish.