Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells
Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells
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DOI：
10.1089/scd.2007.0210
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发表时间：
2008-08-01
影响因子：
4
通讯作者：
You, Seungkwon
中科院分区：
文献类型：
--
作者：
Moon, Jai-Hee;Kwak, Sung Sik;You, Seungkwon
在这项研究中，我们报道了从瘢痕疙瘩头皮组织中分离和鉴定多潜能的瘢痕疙瘩间充质干细胞(KMLSCs)。这些KMLSC在含血清的培养液中培养并在不同的分化介质中诱导分化为脂肪细胞、成骨细胞、软骨细胞、平滑肌细胞和血管生成内皮细胞时，表达典型的间充质干细胞标志蛋白CD13、CD29、CD44、CD90、纤维连接蛋白和波形蛋白。当KMLSC在神经干细胞培养条件下(即在无底物条件下存在表皮生长因子和成纤维细胞生长因子2的情况下)培养时，它们产生了大量含有表达神经脊干细胞标记的巢蛋白、CD133和SOX2阳性细胞的神经球。随后将这些细胞暴露在不同的分化条件下，导致细胞表达神经细胞、星形胶质细胞、少突胶质细胞或雪旺细胞特异性标记。我们的研究表明，KMLSCs可能是再生组织修复和自体移植的替代成体干细胞来源。
In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.