Computer modelling of Tetrahymena axonemes at macromolecular resolution. Interpretation of electron micrographs.
Computer modelling of Tetrahymena axonemes at macromolecular resolution. Interpretation of electron micrographs.
复制标题
大分子分辨率下四膜虫轴丝的计算机建模。
作者：
P. Sugrue;J. Avolio;P. Satir;M. Holwill
提出了在大分子分辨率（4 nm）下四膜虫完整 9+2 纤毛轴丝结构排列的计算机生成模型。该模型协调了负染色、薄片和冷冻断裂电子显微照片中有关子成分的详细信息，将图像整合成一致的三维图片。这说明了一些问题，例如需要压实动力蛋白来形成臂、圆周连接的可视化困难、中央鞘的构造以及内臂和外臂的相对周期性。该模型非常实用，因为随着新信息的出现，它很灵活且易于更改。它对于动态概念的发展也很有用，例如动力蛋白跨桥循环的空间描述（如图所示），或滑动和弯曲过程中相邻双峰之间的关系。
A computer-generated model of the structural arrangement of the complete 9+2 ciliary axoneme of Tetrahymena at macromolecular resolution (4 nm) is presented. The model reconciles detailed information about subcomponents from negative-stained, thin-section and freeze-fracture electron micrographs, integrating the images into a consistent three-dimensional picture. This illuminates problems such as the requirement for compaction of dynein to form the arm, difficulties in visualization of the circumferential links, construction of the central sheath, and the comparative periodicities of the inner and outer arms. The model is pragmatic in that it is flexible and easily changed, as new information becomes available. It is also useful in the development of dynamic concepts, such as a spatial description of the dynein cross-bridge cycle, which is illustrated, or relationships between adjacent doublets during sliding and bending.