Immunoproteomics: the key to discovery of new vaccine antigens against bacterial respiratory infections.
Immunoproteomics: the key to discovery of new vaccine antigens against bacterial respiratory infections.
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DOI：
10.2174/138920312804871184
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发表时间：
2012-12
影响因子：
2.8
通讯作者：
McClean S
中科院分区：
文献类型：
--
作者：
Dennehy R;McClean S
抗生素耐药性的增加和用于预防难治性细菌感染的新型抗菌素的短缺，突显了预防性治疗对预防细菌性病原体感染的重要性。疫苗接种降低了许多严重疾病的发病率，包括呼吸道细菌感染。然而，有许多病原体没有疫苗，有些疫苗对所有年龄组或免疫功能低下的人都无效。免疫蛋白质组学是一项强大的技术，已被用于鉴定潜在的候选疫苗，以防止致病菌。蛋白质组学与使用血清检测免疫反应性抗原的结合突出了感染期间表达的免疫原性蛋白。当患者血清被用作抗原，在人类感染期间促进体液反应时，这是特别有用的。本综述概述了利用免疫蛋白质组学鉴定的候选疫苗的例子，这些候选疫苗在免疫研究中测试时成功地保护了动物免受攻击。许多免疫反应蛋白对几种不相关的病原体是共同的，然而其中一些在动物免疫和挑战研究中并不总是具有保护作用。此外，在免疫蛋白质组学研究中未检测到包括百日咳博德泰拉抗原FHA在内的成熟免疫原的例子，这表明该技术可能不足以代表病原体中的免疫反应蛋白。免疫蛋白质组学是鉴定新型疫苗抗原的一项重要技术，尽管在获得有效的疫苗批准的道路上只有一步之遥。
The increase in antibiotic resistance and the shortage of new antimicrobials to prevent difficult bacterial infections underlines the importance of prophylactic therapies to prevent infection by bacterial pathogens. Vaccination has reduced the incidence of many serious diseases, including respiratory bacterial infections. However, there are many pathogens for which no vaccine is available and some vaccines are not effective among all age groups or among immunocompromised individuals. Immunoproteomics is a powerful technique which has been used to identify potential vaccine candidates to protect against pathogenic bacteria. The combination of proteomics with the detection of immunoreactive antigens using serum highlights immunogenic proteins that are expressed during infection. This is particularly useful when patient serum is used as the antigens that promote a humoral response during human infection are identified. This review outlines examples of vaccine candidates that have been identified using immunoproteomics and have successfully protected animals against challenge when tested in immunisation studies. Many immunoreactive proteins are common to several unrelated pathogens, however some of these are not always protective in animal immunisation and challenge studies. Furthermore, examples of well-established immunogens, including Bordetella pertussis antigen FHA were not detected in immunoproteomics studies, indicating that this technology may underrepresent the immunoreactive proteins in a pathogen. Although only one step in the pathway towards an efficacious approved vaccine, immunoproteomics is an important technology in the identification of novel vaccine antigens.
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影响因子：
6.4
作者：
Hoboth, Christina;Hoffmann, Reinhard;Hogardt, Michael
通讯作者：
Hogardt, Michael
影响因子：
4.6
作者：
Lifshitz, S;Dagan, R;Nebenzahl, YM
通讯作者：
Nebenzahl, YM
影响因子：
3.1
作者：
Fito-Boncompte, Laurene;Chapalain, Annelise;Chevalier, Sylvie
通讯作者：
Chevalier, Sylvie
DOI：
10.1073/pnas.0805532107
发表时间：
2010-02-16
影响因子：
11.1
作者：
Chen, David J.;Osterrieder, Nikolaus;Putnam, David
通讯作者：
Putnam, David
影响因子：
3.1
作者：
GILLELAND, HE;PARKER, MG;BERG, RD
通讯作者：
BERG, RD