Regulation of the activity of the Na+-H+ antiporter in brush-border membrane vesicles from the proximal tubule.
Regulation of the activity of the Na+-H+ antiporter in brush-border membrane vesicles from the proximal tubule.
复制标题
近端小管刷状缘膜囊泡中 Na -H 逆向转运蛋白活性的调节。
DOI：
10.1111/j.1749-6632.1985.tb14868.x
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发表时间：
1985
影响因子：
5.2
通讯作者：
Warnock,DG
中科院分区：
文献类型：
--
作者：
Lowe,A;Lin,HY;Yee,VJ;Warnock,DG
Na+-H+反向转运蛋白存在于许多不同的细胞中，在调节细胞内ph中起着核心作用。反向转运蛋白的激活是对多种生长因子的反应，被认为在细胞生长和分裂的起始中起着重要作用。多种体内生理操作（慢性酸中毒、甲状旁腺切除术、糖皮质激素）通过增加肾近端小管细胞的V来刺激Na+-H+反转运蛋白的活性。z甲状旁腺激素在负鼠肾连续细胞系中降低Na-H逆向转运蛋白的活性。“这些先前的研究已经确定，Na+-H+反转运蛋白活性的调节具有重要的生物学意义。最近的研究表明，修饰位点位于肾Na+-H+反转运蛋白的内部和外部。内部修饰位点可能在Na+-H+反转运蛋白活性的急性调控中起重要作用。本研究的目的是研究可能影响外部修饰位点Na+-H+反转运蛋白的药物的作用。
The Na+-H+ antiporter is present in many different cells, and plays a central role in regulating intracellular pH. Activation of the antiporter occurs in response to a wide variety of growth factors and is thought to play an important role in the initiation of cell growth and division.'A wide variety of in vivo physiologic maneuvers (chronic acidosis, parathyroidectomy, glucocorticoids) stimulate the activity of the Na+-H+ antiporter in renal proximal tubule cells by increasing the V,,,. z Parathyroid hormone decreases the activity of the Na-H antiporter in a continuous cell line from the opossum kidney.'These previous studies have established that the regulation of the activity of the Na+-H+ antiporter is of major biologic importance. Recent studies have demonstrated modifier sites on the internal4 and external5 aspects of the renal Na+-H+ antiporter. It appears that the internal modifier site may play an important role in the acute regulation of Na+-H+ antiporter activity. 6 The purpose of the present studies was to investigate the effects of agents that may affect the Na+-H+ antiporter at the external modifier site.