The metabolism of ferulic acid via 4-vinylguaiacol to vanillin by Enterobacter sp Px6-4 isolated from Vanilla root
The metabolism of ferulic acid via 4-vinylguaiacol to vanillin by Enterobacter sp Px6-4 isolated from Vanilla root
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DOI：
10.1016/j.procbio.2008.06.006
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发表时间：
2008-10-01
影响因子：
4.4
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
--
作者：
Li, Xuemei;Yang, Jinkui;Zhang, Ke-Qin
本研究报告了一种来自香草根的细菌，它具有利用阿魏酸作为唯一碳源来生产香兰素的能力。根据该菌株的16S rDNA序列和生化特征鉴定为肠杆菌Px6-4。以阿魏酸为唯一碳源的主要代谢物包括4-乙烯基愈创木酚、香兰素以及其他相关化学物质，它们都具有相同的愈创木酚碳骨架。根据该菌株培养滤液中非挥发性化合物的结构分析，分离并鉴定了两种可能的新代谢物。这些结果使我们提出了阿魏酸通过4-乙烯愈创木酚转化为香兰素的可能代谢途径。我们的实验首次阐明了阿魏酸在肠杆菌sp. Px6-4中通过脱羧代谢产生4-乙烯基愈创木酚，并进一步转化为香兰素。(C) 2008 Elsevier Ltd版权所有。
This study reports a bacterium from Vanilla roots with the ability to utilize ferulic acid as the sole carbon source to produce vanillin. The strain was identified as Enterobacter sp. Px6-4 based on its 16S rDNA sequence and biochemical characteristics. The primary metabolites using ferulic acid as the sole carbon source include 4-vinylguaiacol, vanillin as well as other related chemicals, all of which share the same guaiacol-carbon-skeleton. Based on structural analyses of non-volatile compounds in the culture filtrate of this strain, two putative novel metabolites were isolated and identified. These results allowed us to propose the possible metabolic pathway leading from ferulic acid to vanillin via 4-vinylguaiacol. Our experiments elucidated for the first time of a ferulic acid metabolism via decarboxylation to produce 4-vinylguaiacol and a further conversion to vanillin in Enterobacter sp. Px6-4. (C) 2008 Elsevier Ltd. All rights reserved.