Facile hydrothermal synthesis of nitrogen, phosphorus-doped fluorescent carbon dots for live/dead bacterial differentiation, cell imaging and two nitrophenols detection
Facile hydrothermal synthesis of nitrogen, phosphorus-doped fluorescent carbon dots for live/dead bacterial differentiation, cell imaging and two nitrophenols detection
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DOI：
10.1016/j.dyepig.2020.108761
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发表时间：
2021
影响因子：
4.5
通讯作者：
Yujiao Tu;Suping Wang;Xiaotian Yuan;Y. Xiang;K. Qin;Yunlin Wei;Qi Zhang;Xuemei Chen;X. Ji
中科院分区：
文献类型：
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作者：
Yujiao Tu;Suping Wang;Xiaotian Yuan;Y. Xiang;K. Qin;Yunlin Wei;Qi Zhang;Xuemei Chen;X. Ji
采用水热法成功制备了以象牙草为原料的氮磷掺杂蓝色荧光碳点（TgN，P-CDs），TgN，P-CDs具有良好的荧光特性，在356激发下，在444 nm处有较强的蓝色荧光发射，具有良好的水溶性，经活/死菌鉴定、细胞成像和洋葱表皮染色，TgN，P-CDs具有较低的生物毒性和良好的生物相容性。此外，纳米探针在检测2，4-二硝基苯酚（2，4-DNP）和4-硝基苯酚（4-NP）时表现出高的选择性和灵敏度，并且在0-50 μM（R2= 0.9923）和0-50 μM（R2= 0.9926）的范围内具有良好的线性，2，4-DNP和4-NP的检测限分别为35.09 nM和78.62 nM。将TgN、P-CDs用于真实的水和土壤样品中4-NP和2，4-DNP的测定，结果满意。结果表明，2，4-DNP和4-NP对TgN、P-CD的荧光猝灭以内过滤效应猝灭为主。为生物成像技术的应用和环境污染物检测提供了新的思路。
The blue fluorescent nitrogen, phosphorus doped carbon dots (TgN,P-CDs) deriving fromThelephoraganbajunZang were successfully obtained by hydrothermal method.TgN,P-CDs possessed excellent fluorescence features and displayed strong blue fluorescence emission at 444 nm under 356 excitation with good water solubility.TgN,P-CDs exhibited low biotoxicity and excellent biocompatibility by live/dead bacterial differentiation, cell imaging and onion epidermal staining. Furthermore, the nanoprobes showed high selectivity and sensitivity in detecting 2,4-dinitrophenol (2,4-DNP) and 4-nitrophenol (4-NP), and excellent linear range of 0–50 μM (R2= 0.9923) and 0–50 μM (R2= 0.9926) with low detection limits of 35.09 nM and 78.62 nM for 2,4-DNP and 4-NP, respectively. TheTgN,P-CDs was successfully applied to determine 4-NP and 2,4-DNP in real water and soil samples. It revealed that the inner filter effect quenching mechanism dominated in quenching ofTgN,P-CDs fluorescent by 2,4-DNP and 4-NP. It provides a new insight on the bioimaging application and environmental pollutant detection.