Evidence that bacteria prefer to adhere to thrombus.
Evidence that bacteria prefer to adhere to thrombus.
复制标题
有证据表明细菌更喜欢粘附在血栓上。
DOI：
10.1097/00002480-199609000-00118
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发表时间：
1996
期刊：
影响因子：
4.2
通讯作者：
S. Mohammad
中科院分区：
文献类型：
--
作者：
H. M. Bos;R. D. de Boer;G. Burns;S. Mohammad
本研究旨在使用体外测试模型来研究血栓形成与感染之间可能存在的关联，其中新鲜牛血通过包含支架样装置的测试导管（3.5 mm内径）进行再循环。调整抗凝剂，使再循环血液在支架上沉积血栓，导致逐渐闭塞，从而阻碍血流。在每个实验中，四个支架样装置被放置在单独的管道中，血液在心脏驱动的心室的帮助下再循环。通过超声波流量检测监测通过这些导管的流量。为了定量细菌与血栓的相互作用，表皮葡萄球菌（15 E10（9））用111铟-oxine标记并加入血液中。实验持续到测试管道中的流量下降到起始流量的10%。在该再循环期间，随着流量逐渐降低，当流量仍为100%时取出一枚支架，第二枚支架为75%，第三枚支架为50%，第四枚支架为10%。通过伽马计数测量与血栓相关的细菌数量。结果表明：（1）在所有实验中，血栓量均随时间的延长而增加（这在单独的实验中通过使用自体111铟标记的血小板得到证实）; 2）血栓大小增加，细菌粘附增加（这通过使用111铟标记的细菌来证实），和3）无论血栓是活的还是用抗生素利福平预处理过的，都发生细菌掺入血栓中。这些观察结果表明，随着血栓的发展，它们可能会优先吸引微生物。这表明具有粘附血栓的器械可能更容易感染。
This study was undertaken to investigate the possible association between thrombosis and infection using an in vitro test model in which fresh bovine blood was recirculated through test conduits (3.5 mm inner diameter) containing stent-like devices. Anticoagulation was adjusted so that the recirculating blood deposited thrombi on the stent to cause gradual occlusion, thus impeding the flow. Four stent-like devices were placed in separate conduits in each experiment, and blood was recirculated with the help of pneumatically driven ventricles. Flow through these conduits was monitored by ultrasonic flow detection. To quantitate bacterial interaction with thrombi, Staphylococcus epidermidis (15E10(9)) was labeled with 111Indium-oxine and added to the blood. Experiments lasted until the flow in the test conduits dropped to 10% of the starting flow. During this recirculation, as flow gradually decreased, one stent was taken out when flow was still at 100%, the second at 75%, the third at 50%, and the fourth at 10% of the starting flow. The number of bacteria associated with the thrombus was measured by gamma counting. The following observations were made: 1) the amount of thrombus increased with time in all experiments (this was confirmed in separate experiments by using autologous 111Indium labeled platelets); 2) bacterial adhesion showed a concomitant increase as thrombus size increased (this was confirmed by using 111Indium labeled bacteria), and 3) bacterial incorporation into the thrombus occurred regardless of whether they were viable or pretreated with the antibiotic rifampin. These observations suggest that as thrombi develop, they may preferentially attract micro-organisms. This suggests that devices with adherent thrombi may have greater susceptibility for infection.