Differentiation Imbalance of Th1/Th17 in Peripheral Blood Mononuclear Cells Might Contribute to Pathogenesis of Hashimoto's Thyroiditis
Differentiation Imbalance of Th1/Th17 in Peripheral Blood Mononuclear Cells Might Contribute to Pathogenesis of Hashimoto's Thyroiditis
复制标题
外周血单个核细胞Th1/Th17分化失衡可能与桥本甲状腺炎发病有关
DOI：
10.1111/j.1365-3083.2010.02425.x
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发表时间：
2010-09-01
影响因子：
3.7
通讯作者：
Xu, H.
中科院分区：
文献类型：
--
作者：
Shi, Y.;Wang, H.;Xu, H.
T辅助17（Th17）细胞是CD4+ T细胞的一个新亚群，可产生促炎细胞因子白介素-17 （IL-17）。Th17细胞最近被证明在许多自身免疫性疾病中发挥关键作用，这些疾病以前被认为是Th1主导的。虽然桥本甲状腺炎（HT）被认为是一种th1型疾病，但Th17细胞在其发病机制中的作用尚不清楚。本研究采用实时定量聚合酶链反应（RT-qPCR）和酶联免疫吸附法（ELISA）检测HT患者外周血单个核细胞（PBMC）和血浆中Th1/Th17细胞相关因子的表达水平。我们的研究结果显示，HT后PBMC中Th1细胞相关T-bet和干扰素γ (ifn - γ) mRNA的表达水平显著降低。然而，Th17相干维甲酸相关孤儿核受体γ t （ROR γ t）和IL-17 mRNA在HT患者中升高。此外，HT患者t -bet与ROR γ t mRNA表达呈负相关，ifn - γ与IL-17的mRNA水平及血浆浓度也出现了类似的现象。提示HT患者中以Th17细胞而非Th1细胞为主，Th17细胞可能参与了HT的发病机制。
T helper 17(Th17) cell is a new subset of CD4+ T cells that produce a proinflammatory cytokine interleukin-17 (IL-17). Th17 cells have recently been shown to play a critical role in many autoimmune diseases that had previously been thought to be Th1 dominant. Although Hashimoto's thyroiditis (HT) was thought to be a Th1-type disease, the contributions of Th17 cells to the pathogenesis remain unclear. In this study, we investigated the expression levels of Th1/Th17 cell-associated factors in peripheral blood mononuclear cells (PBMC) and plasma from patients with HT by quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Our results showed that the expression levels of Th1 cells-related T-bet and interferon-gamma (IFN-gamma) mRNA in PBMC from HT significantly decreased. However, the mRNA of Th17 coherent retinoic acid-related orphan nuclear receptor gamma t (ROR gamma t) and IL-17 in patients with HT increased. In addition, a negative correlation between T-bet and ROR gamma t mRNA expression was found in patients with HT, and the similar phenomena also appeared on the levels of mRNA and plasma concentration between IFN-gamma and IL-17. It suggested that Th17 cells rather than Th1 cells predominated among patients suffering from HT, and Th17 cells might be involved in the pathogenesis of HT.