Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential
Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential
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DOI：
10.3892/or.2011.1192
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发表时间：
2011-05-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Jicheng
中科院分区：
文献类型：
--
作者：
Cui, Hongxia;Lin, Yu;Liu, Jicheng
唾液酸化异常与癌细胞的恶性表型和转移潜能密切相关。然而，乳腺癌中分子的确切性质尚未揭开面纱。在这项研究中，我们研究了50例原发肿瘤和50例配对的淋巴结转移瘤标本以及不同转移潜能的乳腺癌细胞株MDA-MB-231、T-47D和MCF-7中α2，3-唾液酸残基的表达水平。应用组织化学、细胞化学和流式细胞术检测α2，3-唾液酸残基的表达。通过细胞黏附和Transwell实验检测细胞的侵袭和迁移能力。配对配对的淋巴结转移瘤组织中α-2，3-唾液酸残基的表达水平高于原发灶(P=0.0432)。此外，在38例T1/T2期肿瘤中，31例(81.58%)与α2，3-唾液酸残基结合呈弱阳性反应，而12例T3/T4期肿瘤中，仅1例(8.33%)呈弱反应。高转移性乳腺癌细胞株MDA-MB-231与MAL结合最强，α2，3-唾液酸残基的表达水平最高，这取决于α2，3-唾液酸转移酶基因的表达水平。细胞的黏附、侵袭和迁移活性证实，MDA-MB-231细胞对Matrigel具有更强的黏附、迁移和侵袭能力。综上所述，α2，3-唾液酸残基在乳腺癌中的高表达与转移潜能有关。这一特性可能对开发乳腺癌的新治疗方法很重要。
Aberrant sialylation is closely associated with the malignant phenotype of cancer cells and metastatic potential. However, the precise nature of the molecules in breast cancers has not been unveiled. In this study, we investigated the expression levels of alpha 2,3-sialic acid residues of 50 primary tumor cases, 50 pair-matched lymph node metastasis tumor samples and in the MDA-MB-231, T-47D and MCF-7 breast cancer cell lines with different metastatic potential. The expression of alpha 2,3-sialic acid residues was analyzed by histochemistry, cytochemistry and flow cytometry with Maackia amurensis lectin (MAL). The invasion and migration abilities of cells were examined using cell adhesion and transwell in vitro assays. Pair-matched lymph node metastasis tumor samples exhibited higher levels of expression of alpha 2,3-sialic acid residues compared to that of primary tumors (P=0.0432). Furthermore, of 38 tumors cases in T1/T2 stages, 31(81.58%) had weak staining for MAL, which specifically binds to alpha 2,3-sialic acid residues, whereas of 12 tumor cases in T3/T4 stages, only 1 (8.33%) had weak reactions for MAL. The highly metastatic breast cancer cell line MDA-MB-231 exhibited the strongest binding to MAL and the highest expression levels of alpha 2,3-sialic acid residues among the selected cell lines, depending on mRNA expression levels of alpha 2,3-sialyltransferase gene. The adhesion, invasion and migration activities confirmed that MDA-MB-231 exhibited the greater cell adhesion to, migration toward and invasion to Matrigel. Taken together, the high expression of alpha 2,3-sialic acid residues in breast cancer was associated with metastatic potential. This property may be important for developing new therapeutic approaches for breast cancer.