Recent developments in multiplexing techniques for immunohistochemistry.
Recent developments in multiplexing techniques for immunohistochemistry.
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DOI：
10.1586/14737159.2015.1069182
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发表时间：
2015
影响因子：
5.1
通讯作者：
Takayama S
中科院分区：
文献类型：
--
作者：
Dixon AR;Bathany C;Tsuei M;White J;Barald KF;Takayama S
以越来越高的分辨率检测免疫标记分子的方法，即使在低水平时，也是免疫组织化学（IHC）的革命。这些技术对于罕见患者组织标本的管理和检查以及提高早期疾病检测的准确性可能是有价值的。这篇小型综述的目的是强调最近的多路复用方法，这些方法是临床研究中更普遍使用的候选方法，并可能转化为临床。多重免疫组化方法，允许识别至少3个和多达30个离散的抗原，已被分为全切片染色和空间模式化染色类别。还讨论了相关的信号增强技术，可以提高多重IHC检测的性能和通量。本文详述的每种多重IHC技术都与几个优点以及权衡相关，这些优点以及权衡必须被考虑用于正确评估和使用方法。
Methods to detect immuno-labelled molecules at increasingly higher resolution, even when present at low levels, are revolutionizing immunohistochemistry (IHC). These technologies can be valuable for management and examination of rare patient tissue specimens, and for improved accuracy of early disease detection. The purpose of this mini-review is to highlight recent multiplexing methods that are candidates for more prevalent use in clinical research and potential translation to the clinic. Multiplex IHC methods, which permit identification of at least 3 and up to 30 discrete antigens, have been divided into whole section staining and spatially-patterned staining categories. Associated signal enhancement technologies that can enhance performance and throughput of multiplex IHC assays are also discussed. Each multiplex IHC technique, detailed herein, is associated with several advantages as well as tradeoffs that must be taken into consideration for proper evaluation and use of the methods.