Efficacy of ozone on survival and permeability of oral microorganisms
Efficacy of ozone on survival and permeability of oral microorganisms
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DOI：
10.1111/j.1399-302x.2004.00146.x
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发表时间：
2004-08-01
影响因子：
--
通讯作者：
Nishihara, T
中科院分区：
文献类型：
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作者：
Nagayoshi, M;Fukuizumi, T;Nishihara, T
在本研究中，我们研究了臭氧水对口腔微生物和牙菌斑的影响。臭氧水（4 mg/l）处理10 s后几乎未检出微生物。用LIVE/DEAD((R))BacLight（TM）细菌活力试剂盒对细菌细胞进行染色，以评估臭氧水处理后的变形链球菌。荧光显微镜分析显示变形链球菌细胞在臭氧水中瞬间被杀死。电镜观察发现臭氧水处理后的变形链球菌有断裂现象。当实验牙菌斑暴露于臭氧水时，活的变形链球菌数量显著减少。臭氧水对实验性牙菌斑的体外积累有明显的抑制作用。将人类牙菌斑样本暴露在臭氧水中后，几乎没有检测到活菌细胞。这些结果表明，臭氧化水应该有助于减少口腔微生物引起的牙菌斑感染。
In the present study, we examined the effect of ozonated water on oral microorganisms and dental plaque. Almost no microorganisms were detected after being treated with ozonated water (4 mg/l) for 10 s. To estimate the ozonated water-treated Streptococcus mutans, bacterial cells were stained with LIVE/DEAD((R))BacLight(TM) Bacterial Viability Kit. Fluorescence microscopic analysis revealed that S. mutans cells were killed instantaneously in ozonated water. Some breakage of ozonated water-treated S. mutans was found by electron microscopy. When the experimental dental plaque was exposed to ozonated water, the number of viable S. mutans remarkably decreased. Ozonated water strongly inhibited the accumulation of experimental dental plaque in vitro. After the dental plaque samples from human subjects were exposed to ozonated water in vitro, almost no viable bacterial cells were detected. These results suggest that ozonated water should be useful in reducing the infections caused by oral microorganisms in dental plaque.