Voltammetric studies of the interaction of lumazine with cyclodextrins and DNA
Voltammetric studies of the interaction of lumazine with cyclodextrins and DNA
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DOI：
10.1016/s0731-7085(01)00624-0
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发表时间：
2002-04-15
影响因子：
3.4
通讯作者：
Al-Nayeli, AA
中科院分区：
文献类型：
--
作者：
Ibrahim, MS;Shehatta, IS;Al-Nayeli, AA
用差示脉冲溶出伏安法、循环伏安法和紫外-可见光谱研究了水溶液中抗菌药物鲁马津与α，P-环糊精和DNA的相互作用。电化学和吸收光谱数据表明鲁马津与α-、β-环糊精和DNA形成1：1的络合物。这一过程发生在悬汞电极上，发现鲁马津分子的络合作用增强了堆积作用，这可能有助于在电极表面形成垂直堆积的鲁马津-α-环糊精络合物。根据电流或吸光度的变化，确定了这些络合物的生成常数，进而确定了它们的吉布斯能。α-环糊精较小的空腔对鲁马津的亲和力大于对β-环糊精的亲和力。此外，还用伏安法研究了鲁马津-α-环糊精或鲁马津-对环糊精包合物与DNA的相互作用。结果表明，鲁马津对DNA具有较高的亲和力，包合物在与DNA结合时发生解离。(C)2002 Elsevier Science B.V.保留所有权利。
The interaction of lumazine, an antibacterial drug, with alpha-, P-cyclodextrins and DNA in aqueous solution was studied by differential pulse stripping voltammetry and cyclic voltammetry as well as UV-vis spectroscopy. The electrochemical and absorption spectral data indicated a 1:1 complex formation of lumazine with alpha-, beta-cyclodextrins and DNA. The nature of the process, taking place at the hanging mercury drop electrode, was clarified, It was found that the complexation of lumazine molecules enhances the stacking interactions which might facilitate the formation of a perpendicularly stacked layer of lumazine-alpha-cyclodextrin complex on the electrode surface. Based on the variations in the current or absorbance, the formation constants and consequently, the Gibbs energy of these complexes were determined. The small size cavity of a-cyclodextrin was found to have a greater affinity for lumazine than the beta-cyclodextrin. Moreover, the interactions of lumazine-alpha-cyclodextrin or lumazine-p-cyclodextrin inclusion complex with DNA have been investigated by means of voltammetry. The results suggest that lumazine displayed high affinity for DNA and the inclusion complex decomposed when it binds to DNA. (C) 2002 Elsevier Science B.V. All rights reserved.