GROWTH AND CELL-KINETICS OF HUMAN HAIR PAPILLA CELLS-INVITRO - AN AUTORADIOGRAPHIC AND FLOW CYTOMETRIC STUDY
GROWTH AND CELL-KINETICS OF HUMAN HAIR PAPILLA CELLS-INVITRO - AN AUTORADIOGRAPHIC AND FLOW CYTOMETRIC STUDY
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DOI：
10.1111/j.1365-2184.1991.tb01165.x
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发表时间：
1991-07-01
影响因子：
8.5
通讯作者：
HORNSTEIN, OP
中科院分区：
文献类型：
--
作者：
BASSUKAS, ID;ARAI, A;HORNSTEIN, OP
毛乳头是一种特殊的真皮隔膜，在毛发生长的生物学中起着重要的作用。近年来，毛乳头细胞(HPC)被认为是影响毛发生长的药物的可能靶点。HPC的分离和培养有助于对此类药物的筛选。本工作对人枕部头皮毛囊HPC的体外生长和细胞动力学进行了研究。HPC按照Gompertz函数生长，即在形成融合培养之前有相当长的生长延迟，可能是由于潜在倍增时间(T(POT))的延长和细胞损失率的平行增加。HPC的[H-3]dt标记指数强烈依赖于继代培养的年龄，周期约为4天。通过[H-3]dT连续标记和流式细胞术的联合应用，发现细胞的潜在倍增时间约为93h，表明生长分数(GF)=1，S期和G2+M期的持续时间约为8h。
Hair papilla, a distinct specialized dermal compartment, plays a fundamental role in the biology of hair growth. Recently some attention has been focused on hair papilla cells (HPC) as possible targets for drugs influencing the hair growth. Isolation and cultivation of the HPC facilitates screening for such drugs. In the present work, growth and cell kinetics of human occipital scalp follicle HPC have been studied in vitro. HPC grow according to a Gompertz function, i.e. with considerable growth delay long before becoming confluent cultures, due probably to elongation of the potential doubling time (T(pot)) and to a parallel increasing cell loss rate. The [H-3]dT labelling index of the HPC strongly depends on the age of the subculture; the cycle time being about 4 days. A potential doubling time of about 93 h, indicative of growth fraction (GF) = 1, and a duration of S phase and G2 + M phase of about 8 h each were found by the combined application of continuous labelling with [H-3]dT and DNA flow cytometry.