Components of human papillomavirus that activate transcription and support plasmid replication in human airway cells.
Components of human papillomavirus that activate transcription and support plasmid replication in human airway cells.
复制标题
人乳头瘤病毒的成分，可在人呼吸道细胞中激活转录并支持质粒复制。
DOI：
10.1165/ajrcmb.20.5.3479
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Chow,LT
中科院分区：
文献类型：
--
作者：
Gadi,VK;Zou,N;Liu,JS;Cheng,S;Broker,TR;Sorscher,EJ;Chow,LT
人乳头瘤病毒（HPV），如6型和11型，可在患者的气道上皮细胞中建立终身感染，长期感染可导致肺部受累和死亡。这种持久性的机制取决于病毒增强子和启动子的转录活性以及该病毒在感染组织中维持其双链环状DNA基因组的能力。我们研究了人类呼吸道细胞系中HPV序列元件和蛋白产物的转录和复制特性。我们发现，上游调控区的掺入和两个病毒编码的蛋白质，E1和E2的表达载体的共转染，赋予约5,000倍的刺激报告基因的表达。通过改良的瞬时复制试验证明了转染的人气道细胞和FVB/N-C57 BL/6小鼠肺中的瞬时质粒复制。 这些结果对呼吸道细胞中的病毒发病机制和HPV复制子将基因转移到呼吸道上皮细胞中的潜力具有重要意义。
Human papillomaviruses (HPVs) such as types 6 and 11 can establish lifelong infections in airway epithelial cells in patients, and long-term infection can lead to pulmonary involvement and death. The mechanisms underlying this persistence depend on both the transcriptional activity of the viral enhancers and promoters and the ability of this virus to maintain its double-stranded circular DNA genome in infected tissues. We investigated the transcription and replication properties of HPV sequence elements and protein products in a human airway cell line. We showed that incorporation of the upstream regulatory region and cotransfection with expression vectors of two virus-encoded proteins, E1 and E2, conferred approximately 5,000-fold stimulation of reporter gene expression. Transient plasmid replication in transfected human airway cells and lungs of FVB/N-C57BL/6 mice was demonstrated by a modified transient replication assay. These results have important implications for viral pathogenesis in airway cells and the potential of HPV-based replicons for gene transfer into airway epithelium.