A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex
A microsatellite-based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex
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DOI：
10.1111/j.1471-8286.2004.00869.x
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发表时间：
2005-03-01
期刊：
影响因子：
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通讯作者：
Christiansen, DG
中科院分区：
文献类型：
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作者：
Christiansen, DG
中国林蛙（Rana esculenta）是中国林蛙（Rana lessonae，LL）和中国林蛙（Rana ridiatra，RR）的杂交种，杂交种可以是二倍体（LR）或三倍体（LLR或LRR）。基因型可以从成年青蛙的红细胞大小和形态学中粗略地确定，但准确的基因型需要更费力的方法。在这里，我证明了L和R基因组具有特定的微卫星等位基因，基因型和倍性可以准确地推断出PCR扩增（聚合酶链反应扩增）基因组特异性等位基因的定量比率。这种方法大大方便了R. esculenta复杂，并允许分析保存不良的样品和胚胎。
Rana esculenta is a hybrid between Rana lessonae (LL) and Rana ridibunda (RR), and hybrids may be diploid (LR) or triploid (LLR or LRR). Genotypes can be roughly determined from erythrocyte size and morphometry in adult frogs, but accurate genotyping requires more labourious methods. Here I demonstrate that both the L and R genomes have specific microsatellite alleles, and that genotype and ploidy can lie accurately inferred from the quantitative ratio of PCR-amplified (polymerase chain reaction-amplified) genome-specific alleles. This method greatly facilitates genotyping in DNA studies of the R. esculenta complex and allows analysis of badly preserved samples and embryos.