An HPLC-MS/MS method for simultaneous determination of decitabine and its valyl prodrug valdecitabine in rat plasma
An HPLC-MS/MS method for simultaneous determination of decitabine and its valyl prodrug valdecitabine in rat plasma
复制标题
HPLC-MS/MS同时测定大鼠血浆中地西他滨及其缬氨酰前药伐地他滨的含量。
DOI：
10.1016/j.jchromb.2012.12.040
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发表时间：
2013-02-15
影响因子：
3
通讯作者：
He, Zhonggui
中科院分区：
文献类型：
--
作者：
Zhang, Youxi;Sun, Jin;He, Zhonggui
建立了同时测定大鼠血浆中地西他滨和伐地他滨浓度的HPLC-MS/MS方法。分析物在C-18柱（150 mm × 4.6 mm，3.5 μ m）上分离，并采用配备电喷雾电离（ESI）源的三重四极杆质谱仪进行检测。采用阳离子交换固相萃取法从大鼠血浆中提取地西他滨，回收率>82%。校准曲线在地西他滨10- 10，000 ng/mL和伐地他滨5-500 ng/mL的浓度范围内呈线性。地西他滨和伐地他滨的定量下限（LLOQ）分别为10和5 ng/mL。日内和日间精密度均小于15%，相对误差（RE）均在± 15%以内。经验证的方法被成功地应用于大鼠的药代动力学研究后，无论是地西他滨或伐地西他滨口服给药的Sprague-Dawley大鼠。(c)2013爱思唯尔有限公司版权所有。
A simple and sensitive HPLC-MS/MS method was developed and validated for the simultaneous determination of decitabine and valdecitabine in rat plasma. The analytes were separated on a C-18 column (150 mm x 4.6 mm, 3.5 mu m) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was applied for detection. A clean solid-phase extraction procedure with cation exchange cartridge was employed to extract the analytes from rat plasma with high recovery of decitabine (>82%). The calibration curves were linear over a concentration range of 10-10,000 ng/mL for decitabine and 5-500 ng/mL for valdecitabine. The lower limit of quantitation (LLOQ) of decitabine and valdecitabine was 10 and 5 ng/mL, respectively. The intra-day and inter-day precisions were less than 15% and the relative error (RE) was all within +/- 15%. The validated method was successfully applied to a pharmacokinetics study in rats after either decitabine or valdecitabine orally administrated to the Sprague-Dawley rats. (c) 2013 Elsevier B.V. All rights reserved.