Preparation and characterization of alginate microspheres containing a model antigen
Preparation and characterization of alginate microspheres containing a model antigen
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DOI：
10.1016/s0378-5173(98)00303-2
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发表时间：
1998-12-30
影响因子：
5.8
通讯作者：
Préat, V
中科院分区：
文献类型：
--
作者：
Lemoine, D;Wauters, F;Préat, V
本研究的目的是评估使用海藻酸盐微球递送抗原的技术可行性。该微球采用乳化法制备，具有抗原递送系统的特点。选择合适的参数制备海藻酸盐微球，平均直径为8 μ m。选择牛血清白蛋白（BSA）作为模型抗原，其包封率和载药量都很高（分别为90%和10% w/w）。包封过程不影响包封抗原的分子量和抗原性。体外释放谱显示，包封的牛血清白蛋白释放速度快，特别是在磷酸盐缓冲盐水溶液中。然而，用聚赖氨酸包被海藻酸盐微球或用较高的海藻酸盐分子量制备海藻酸盐微球时，其释放速率降低。因此，海藻酸盐微球在技术上是一种很有前途的抗原递送系统。(C) 1998 Elsevier Science B.V.版权所有
The goal of this study was to evaluate the technological feasibility of delivering antigen using alginate microspheres. The microspheres were prepared by an emulsification technique and fully characterized as antigen delivery system. Selection of appropriate parameters enabled the preparation of alginate microspheres with a mean diameter of 8 mu m. The encapsulation efficiency of bovine serum albumin (BSA), chosen as model antigen, as well as the BSA loading were very high (>90% and 10% w/w, respectively). The process of encapsulation did not affect the molecular weight or the antigenicity of the entrapped antigen. The in vitro release profile showed a fast release rate of encapsulated BSA, particularly in phosphate buffered saline solution. However, a decrease of the release rate was observed when alginate microspheres were coated with poly(L-lysine) or prepared with higher alginate molecular weight. Therefore, alginate microspheres appear, technologically, a promising antigen delivery system. (C) 1998 Elsevier Science B.V. All rights reserved.