Molecular Design of Ultrabright Semiconducting Polymer Dots with High NIR-II Fluorescence for 3D Tumor Mapping
Molecular Design of Ultrabright Semiconducting Polymer Dots with High NIR-II Fluorescence for 3D Tumor Mapping
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DOI：
10.1002/adhm.202100993
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发表时间：
2021-09-22
影响因子：
10
通讯作者：
Chan, Yang-Hsiang
中科院分区：
文献类型：
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作者：
Li, Yi-Xuan;Su, Shih-Po;Chan, Yang-Hsiang
荧光探针在第二个近红外（NIR-II, 1000-1700 nm）窗口发射，具有在哺乳动物中进行深层组织成像的能力，预示着外科方法的新时代。然而，由于这些NIR-II探针的荧光量子产率（QYs）较低，其亮度仍然远远不能令人满意，从而妨碍了对全器官血管网络等高分辨率图像的实时观察。本文描述了一系列半导体聚合物点（Pdots）的分子工程，这些点与聚集诱导的发射部分结合在一起，在NIR-II窗口中显示出高达14%的qy。利用超高亮度，利用1400 nm长通滤光片实现了小鼠体内三维肿瘤定位。为了进一步了解半导体聚合物的几何结构和电子结构如何影响其光学性质，进行了深入而彻底的密度泛函理论计算来解释实验结果。该研究为进一步设计高亮度NIR-II Pdots奠定了基础。
Fluorescence probes emitting in the second near-infrared (NIR-II, 1000-1700 nm) window with the ability for deep-tissue imaging in mammals herald a new era in surgical methodology. However, the brightness of these NIR-II probes is still far from satisfactory due to their low fluorescence quantum yields (QYs), preventing the observation of high-resolution images such as whole-organ vascular networks in real time. Described here is the molecular engineering of a series of semiconducting polymer dots (Pdots) incorporated with aggregation-induced emission moieties to exhibit the QYs as high as 14% in the NIR-II window. Benefiting from the ultrahigh brightness, a 1400 nm long-pass filter is utilized to realize in vivo 3D tumor mapping in mice. To further understand how the geometrical and electron structures of the semiconducting polymers affect their optical properties, the in-depth and thorough density-functional theory calculations are performed to interpret the experimental results. This study lays the groundwork for further molecular design of highly bright NIR-II Pdots.