STAINED PECTIN AS SEEN IN THE ELECTRON MICROSCOPE
STAINED PECTIN AS SEEN IN THE ELECTRON MICROSCOPE
复制标题
电子显微镜中看到的染色果胶
DOI：
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发表时间：
1960
期刊：
影响因子：
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通讯作者：
A. Frey
中科院分区：
文献类型：
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作者：
P. Albersheim;K. Mühlethaler;A. Frey
本文报道了果胶在植物幼细胞中分布的电镜研究。使用1至3 mm长的深色洋葱根。为了使果胶物质选择性地对电子致密，它们首先与碱性羟胺反应。该处理产生果胶异羟肟酸，其又用铁离子处理以形成不溶性复合物。将组织包埋、切片，然后通过电子显微镜观察。致密的沉积物的铁被发现在该地区的中层和第二个地区附近的表面的初级壁。观察到不同成熟度的横壁。更频繁的未成熟横壁的果胶直接进入腋（纵）壁的内反应层。另一方面，更成熟的横壁的果胶与腋壁的中层果胶密切相关。结果表明，果胶的中间层代表的果胶物质的热水可溶性部分，而腋壁和横壁果胶的内层代表所谓的残余馏分。热提取法基本上可以去除所有的电子密度高的物质。
This paper describes electron microscopic studies on the distribution of pectin within young plant cells. Dark-grown onion roots, from 1 to 3 mm. in length, were used. In order to make the pectic substances selectively dense to electrons, they were first reacted with basic hydroxylamine. This treatment produces pectic hydroxamic acids, which in turn were treated with ferric ion to form insoluble complexes. The tissue was imbedded, sectioned, and then observed by electron microscopy. Dense deposits of iron were found in the region of the middle lamella and in a second area near the surface of the primary wall. Transverse walls of varying maturity were noted. The pectin of the more frequent, immature cross-walls, leads directly into the inner reacting layer of the axillary (longitudinal) wall. The pectin of the more mature transverse walls becomes, on the other hand, intimately associated with the middle lamella pectin of the axillary wall. It is shown that the pectin of the middle lamella represents the hot water-soluble portion of the pectic substance, while the internal layer of the axillary wall and the transverse wall pectin represent the so called residual fraction. Hot versene extraction removes essentially all electron-dense material.