The viability of hamster spermatozoa stored in the isthmus of the oviduct: the importance of sperm-epithelium contact for sperm survival.
The viability of hamster spermatozoa stored in the isthmus of the oviduct: the importance of sperm-epithelium contact for sperm survival.
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DOI：
10.1095/biolreprod42.3.450
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发表时间：
1990-03
影响因子：
3.6
通讯作者：
T. T. Smith-T.;Ryuzo Yanagimachi
中科院分区：
文献类型：
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作者：
T. T. Smith-T.;Ryuzo Yanagimachi
当仓鼠在发情期开始后不久交配时，精子在排卵前储存在输卵管下部（峡部）。本研究旨在确定峡部精子存活至受精的比例。雌性在排卵前5至6.5小时交配。当在交配开始后2 h通过切除的输卵管壁观察峡部中的精子时，在管腔中观察到精子游离，附着在壁的粘膜表面和隐窝中。管腔中的绝大多数精子是不动的，而大多数附着在壁的粘膜表面的精子和几乎所有在隐窝中的精子都表现出鞭毛运动。这表明附着在粘膜上和/或储存在隐窝中有利于精子的存活。在交配后不同时间（2-8 h）连续冲洗输卵管，以从管腔（第一次冲洗）、粘膜表面（第二次冲洗）和隐窝（第三次冲洗）中清除精子。第一次冲洗时精子数量最多，第二次冲洗次之，第三次冲洗最少。当在冲洗介质中加入台盼蓝以区分活精子和死精子时，无论交配后的时间如何，第一次冲洗回收的肝精子百分比最低（2-22%），第二次冲洗略高（16-37%），第三次冲洗回收的肝精子百分比最高（51-69%）。这些数据表明，大多数储存在仓鼠峡部的精子在排卵前死亡。（250字处删节）
When hamsters mate shortly after the onset of estrus, spermatozoa are stored in the lower oviduct (isthmus) during the preovulatory period. The present study was performed to determine what proportion of the spermatozoa in the isthmus survive until fertilization. Females were mated 5 to 6.5 h before ovulation. When spermatozoa in the isthmus were observed through the wall of oviducts excised 2 h after the onset of mating, spermatozoa were seen free in the lumen, attached to the mucosal surface of the wall, and in crypts. The vast majority of spermatozoa in the lumen were immotile, whereas most of those attached to the mucosal surface of the wall and almost all of the those in the crypts exhibited flagellar movement. This suggested that attachment to the mucosa and/or storage in the crypts is beneficial to the survival of spermatozoa. Sequential flushing of an oviduct at various times (2-8 h) after mating was used to remove spermatozoa from the lumen (first flush), from the mucosal surface (second flush), and from the crypts (third flush). The highest number of spermatozoa was always contained in the first flush, the next highest in the second flush, and the smallest in the third flush. When Trypan blue was included in the flushing medium to differentiate live and dead spermatozoa, the first flush recovered the smallest percentage of liver spermatozoa (2-22%), the second flush slightly more (16-37%), and the third flush the highest (51-69%), regardless of the time after mating. These data indicate that the majority of spermatozoa stored in the hamster isthmus die before ovulation.(ABSTRACT TRUNCATED AT 250 WORDS)