Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers
Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers
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DOI：
10.5713/ajas.2004.445
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发表时间：
2004-04-01
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影响因子：
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通讯作者：
Rege, JEO
中科院分区：
文献类型：
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作者：
Chenyambuga, SW;Hanotte, O;Rege, JEO
利用19个微卫星标记对撒哈拉以南非洲山羊的遗传多样性进行了评估。从东非（马赛、基盖齐、穆本德、西北高地、阿尔西-巴莱）、南部非洲（恩德贝莱、帕富里）和西非（西非侏儒、Maure、Djallonke）取样。欧洲品种（Grisons条纹，Toggenburg），亚洲品种（蒙古羊绒，Bandipur）和中东品种（阿拉伯）也包括在内。每个位点的平均等位基因数和平均基因多样性分别为5.26+/-0.464（Djallonke）至7.05+/-0.516（Mubende）和0.542+/-0.036（Pafuri）至0.672+/-0.031（Ndebele）。用G（ST）和theta估计的品种间变异分别占总遗传变异的14.6%（theta）和15.7%（G（ST））。品种间遗传距离的D-A测度结果表明，Pafuri和Djallonke品种间的遗传距离最大，Arsi-Bale和North-West Highland品种间的遗传距离最小。一个邻居加入树的品种关系显示，品种分组根据其地理起源。主成分分析支持品种的分组，根据其地理来源。得出的结论是，撒哈拉以南非洲山羊品种的关系是根据其地理位置，这意味着非洲东部，西非和南部非洲的山羊是遗传上不同的。在每个亚区域内，山羊种群可以根据形态特征进行区分。
Genetic diversity of sub-Saharan African goats was assessed using 19 microsatellite markers. Breeds were sampled from eastern Africa (Maasai, Kigezi, Mubende, North West Highland, Arsi-Bale), southern Africa (Ndebele, Pafuri) and West Africa (West African Dwarf, Maure, Djallonke). European breeds (Grisons Striped, Toggenburg), Asian breeds (Mongolian Cashmere, Bandipur) and a Middle East breed (Arab) were also included. The mean number of alleles per locus and average gene diversity ranged from 5.26+/-0.464 (Djallonke) to 7.05+/-0.516 (Mubende) and from 0.542+/-0.036 (Pafuri) to 0.672+/-0.031 (Ndebele), respectively. The between breeds variation evaluated using G(ST) and theta were found to account for 14.6% (theta) and 15.7% (G(ST)) of the total genetic variation. The D-A measure of genetic distance between pairs of breeds indicated that the largest genetic distance was between Pafuri and Djallonke while the lowest genetic distance was between Arsi-Bale and North West Highland. A neighbour-joining tree of breed relationships revealed that the breeds were grouped according to their geographic origins. Principal component analysis supported the grouping of the breeds according to their geographic origins. It was concluded that the relationships of sub-Saharan African goat breeds were according to their geographical locations implying that the goats of eastern Africa, West Africa and southern Africa are genetically distinct. Within each sub-region, goat populations could be differentiated according to morphological characteristics.