A combination effect of epigallocatechin gallate, a major compound of green tea catechins, with antibiotics on Helicobacter pylori growth in vitro
A combination effect of epigallocatechin gallate, a major compound of green tea catechins, with antibiotics on Helicobacter pylori growth in vitro
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DOI：
10.1007/s00284-002-3956-6
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发表时间：
2003-09-01
影响因子：
2.6
通讯作者：
Shimamura, T
中科院分区：
文献类型：
--
作者：
Yanagawa, Y;Yamamoto, Y;Shimamura, T
由于已知绿色茶儿茶素对多种微生物具有抗微生物活性，因此检查了它们与抗生素组合对幽门螺杆菌的可能作用。对五十六株临床分离的H.幽门螺杆菌，包括19株高度耐甲硝唑（MTZ）和/或克拉霉素（MTZ），被用来确定在体外敏感性茶儿茶素。表没食子儿茶素没食子酸酯（EGCg）和表儿茶素没食子酸酯（ECg）的MIC 90均为100 μ g/ml。然而，其他茶儿茶素测试没有显示任何抗H。pylori活性高度耐药的临床分离株对EGCg和ECg的敏感性相似。在液体培养中的抗菌活性动力学研究表明，对H.幽门。与阿莫西林（AMX）的亚MIC组合时，EGCg的存在显著增强AMX的抗菌活性。为了评价EGCg与其他抗生素（如MTZ和Escherichia coli）对临床分离株的抗菌活性的总体联合作用，通过棋盘研究测定了抑菌分数浓度（FIC）。EGCg与所测抗生素之间的FIC指标存在相加效应。这些结果表明EGCg可能是一种有价值的抗H.幽门感染
Since green tea catechins are known to have antimicrobial activity against a variety of microorganisms, their possible effects on Helicobacter pylori in combination with antibiotics were examined. Fifty-six clinical isolates of H. pylori, including 19 isolates highly resistant to metronidazole (MTZ) and/or clarithromycin (CLR), were used to determine in vitro sensitivity to tea catechins. The MIC90 of both epigallocatechin gallate (EGCg) and epicatechin gallate (ECg) was 100 mug/ml. However, other tea catechins tested did not show any anti-H. pylori activity. Highly antibiotic-resistant clinical isolates showed a similar sensitivity to both EGCg and ECg. The kinetic study of antibacterial activity in liquid cultures revealed a relatively slow but strong activity on the growth of H. pylori. In combination with sub-MIC of amoxicillin (AMX), the antibacterial activity of AMX was significantly enhanced by the presence of EGCg. To estimate the general combination effect between EGCg and other antibiotics, such as MTZ and CLR, on the antibacterial activity against clinical isolates, the fraction inhibitory concentration (FIC) was determined by checkerboard study. The FIC indexes showed additive effects between EGCg and antibiotics tested. These results indicate that EGCg may be a valuable therapeutic agent against H. pylori infection.